尾穗苋甲醇提取物对扑热息痛致大鼠肝损伤的保护作用(英文)  

Hepatoprotective activity of methanol extract of Amaranthus caudatus Linn. against paracetamol-induced hepatic injury in rats

作　　者：Bagepalli Srinivas Ashok Kumar Kuruba Lakshman Peresandra Avalakondarayppa Arun Kumar Gollapalle Lakshminarayana Shastry Viswantha Veeresh Prabhakar Veerapur Boreddy Shivanadppa Thippeswamy Bachappa Manoj 

机构地区：[1]Department of Pharmacognosy,Sri K.V.College of Pharmacy,Chickballapur [2]Department of Pharmacognosy,PES College of Pharmacy,Bangalore [3]Deparment of Pharmcology,Sri K.V.College of Pharmacy,Chickballapur [4]Department in Quality Assurance,Sree Siddaganga College of Pharmacy [5]Department of Pharmacology,Sree Siddaganga College of Pharmacy [6]Department of Pharmaceutical Chemistry,Sri K.V.College of Pharmacy,Chickballapur

出　　处：《中西医结合学报》2011年第2期194-200,共7页Journal of Chinese Integrative Medicine

摘　　要：目的:检测尾穗苋(Amaranthus caudatus)甲醇提取物对扑热息痛致大鼠肝损伤的保护作用。方法:5组大鼠分别给予羧甲基纤维素钠、扑热息痛、尾穗苋甲醇提取物(200或400mg/kg)并扑热息痛、水飞蓟素并扑热息痛。给药14d后采集大鼠血样进行血液生化检查,而后处死大鼠,收集各组大鼠肝脏组织样本供组织病理学检查。检测的指标包括肝功能标志物如丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、血清白蛋白(albumin,ALB)、总蛋白(total protein,TP)、总胆红素(total bilirubin,TB)和直接胆红素(direct bilirubin,DB)及氧化损伤标志物如丙二醛(malondialdehyde,MDA)、还原型谷胱甘肽(reduced glutathione,GSH)、总硫醇(total thiols,TT)和过氧化氢酶(catalase,CAT)。结果:与未治疗组相比,浓度为200和400mg/kg的尾穗苋甲醇提取物均能显著改善由扑热息痛引起的大鼠肝损伤,改善扑热息痛导致的ALT、AST、TB和DB水平升高及血清ALB水平降低(P<0.01)。与未治疗组相比,浓度为200和400mg/kg的尾穗苋甲醇提取物能够显著降低扑热息痛导致的MDA及TP水平增高(P<0.01),并显著升高GSH、CAT及TT水平(P<0.01)。大鼠肝脏组织病理学检查的结果也证实了尾穗苋甲醇提取物对大鼠肝脏的保护作用。结论:本研究证实了尾穗苋的甲醇提取物对于扑热息痛引起的大鼠肝损伤具有显著的保护作用,证实了其在民族医学中治疗肝病的药用价值。Objective： Methanol extract of whole plant of Amaranthus caudatus （MEAC） was screened for hepatoprotective potency against paracetamol （PCM）-induced liver damage in Wistar rats. Methods ： Rats of five groups were given sodium carboxy methyl cellulose, PCM, MEAC （200 and 400 mg/kg, respectively） plus PCM, and silymarin plus PCM, respectively. Fourteen days after administration, blood samples were collected for biochemical estimation, then animals were sacrificed and liver samples were collected, weighed and subjected for histopathological studies. Liver marker enzymes such as serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） and serum albumin （ALB）, total protein （TP）, total bilirubin （TB） and direct bilirubin （E）B） levels and the markers for oxidative defense namely malondialdehyde （MDA）, reduced glutathione （GSH）, total thiols （TT） and catalase （CAT） were measured. Results： MEAC at 200 and 400 mg/kg significantly normalized the PCM-induced biochemical changes compared with PCM-treated group （P^0. 01）; increased ALT, AST, TB and DB levels and decreased serum ALB were significantly reversed by the MEAC treatment （200 and 4：00 mg/kg）. Treatment with MEAC （200 and 400 mg/kg） significantly prevented the rise of MDA and TP levels （P〈0.01）, and prevented the reduction of GSH, CAT and TT levels significantly compared with PCM-treated group （P〈0. 01）. Histopathological examination of the liver sections also proved the hepatoprotective activity of MEAC. Conclusion： MEAC shows significant hepatoprotective activity against PCM-induced liver damage in rats. This finding supports the use of the plant in ethnomedicine for the treatment of liver diseases.

关 键 词：苋属 植物提取物 保肝药 扑热息痛 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]