黄连、吴茱萸诱导人胃癌细胞SGC-7901凋亡的配比研究  

Proportion of Coptidis rhizoma and Evodiae fructus in the compound preparation:its effect in inducing apoptosis of SGC-7901 cells

作　　者：彭求贤[1] 杨大坚 史珏[3] 蔡红兵[1] 莫志贤[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]深圳市中药药学与分子药理学研究重点实验室,广东深圳518057 [3]香港浸会大学系统生物学中心

出　　处：《南方医科大学学报》2011年第2期226-229,共4页Journal of Southern Medical University

基　　金：973计划前期研究专项(2006CB708516)~~

摘　　要：目的探讨黄连、吴茱萸不同配比的水提物(CREF)对人胃癌细胞(SGC-7901)生长和凋亡的影响,找出两药的最佳配比。方法采用MTT比色法观察不同配比的CREF对SGC-7901细胞生长的影响。应用流式细胞仪检测细胞凋亡率和细胞线粒体膜电位的变化。结果 1 mg/ml不同配比的CREF作用SGC-7901细胞24 h和48 h后,对细胞生长有明显抑制作用(P<0.05),且细胞的抑制率与黄连的剂量呈正比。流式细胞仪PI单染亚二倍体峰分析结果显示,以1 mg/mL不同配比的CREF 1∶6、2∶5、3∶4、4∶3、5∶2、6∶1(黄连:吴茱萸)作用于SGC-7901细胞48 h后,细胞凋亡率分别为(8.50±1.59)%、(9.90±1.01)%、(17.15±1.68)%、(21.55±1.97)%、(34.10±1.06)%和(34.40±1.02)%,与对照组(1.69±1.91)%比较,差异均有统计学意义(P<0.01)。JC-1染色检测结果显示,不同配比的CREF作用于SGC-7901细胞6 h后,绿/红荧光比值显著降低,细胞线粒体膜电位下降。结论 CREF能明显抑制SGC-7901细胞生长并可诱导其凋亡,黄连:吴茱萸最佳配比为6∶1。Objective To investigate the effect of water extracts of Coptidis Rhizoma and Evodiae Fructus（CREF） on the proliferation and apoptosis of human gastric carcinoma cells（SGC-7901） and determine the optimal proportion of Coptidis rhizoma to Evodiae fructus.Methods The growth inhibition of SGC-7901 cells treated with the water extracts of CREF of varying proportions was tested with MTT assay.The cell apoptotic rate and mitochondrial membrane potential were analyzed with flow cytometry.Results The water extract of CREF with Coptidis Rhizoma： Evodiae Fructus proportions at 1∶6,2∶5,3∶4,4∶3,5∶2,and 6：1 all significantly inhibited the growth of SGC-7901 cells after a 24-h or 48-h treatment（P0.05）.The growth inhibition and cell death ratio both exhibited a dose-dependent pattern of Coptidis Rhizoma.Flow cytometry analysis showed that,after treatment of the cells with CREF at the proportions of 1∶6,2∶5,3∶4,4∶3,5∶2,and 6∶1,the apoptotic rate were（8.50±1.59）%,（9.90±1.01）%,（17.15±1.68）%,（21.55±1.97）%,（34.10±1.06）% and（34.40±1.02）%,respectively,all significantly higher than that in the control group [（1.69±1.91）%,P0.05].JC-1 Kit staining showed that mitochondrial membrane potential of SGC-7901 cells was decreased and the ratio of green to red fluorescence increased significantly after incubation with CREF.Conclusion CREF can inhibit the growth and induce apoptosis of SGC-7901 cells,and the strongest effect is achieved at the optimal proportion of Coptidis Rhizoma and Evodiae Fructus at 6∶1.

关 键 词：黄连 吴茱萸 配伍比率 细胞凋亡 线粒体膜电位 

分 类 号：R285.5[医药卫生—中药学]