长期低剂量牙龈卟啉单胞菌感染对脂蛋白基因敲除小鼠动脉粥样硬化形成的影响  

Effect of longterm lower Porphyromonas gingivalis infection on the progression of atherosclerosis in apolipoprotein E-knocked out mice

作　　者：汤旭娜[1] 吴娟[1] 孙卫斌[1] 

机构地区：[1]南京大学口腔医学院·南京市口腔医院牙周科,210008

出　　处：《中华口腔医学杂志》2011年第2期75-78,共4页Chinese Journal of Stomatology

基　　金：基金项目：江苏省“六大人才高峰”第五批高层次人才项目资助计划[苏人通（2008）239号]

摘　　要：目的 评价牙龈卟啉单胞菌（Porphyromonas gingivalis,Pg）口腔内低剂量、长期、多次接种是否可影响载脂蛋白基因敲除小鼠（apolipoprotein E-knocked out,ApoE-/-）的动脉粥样硬化（atherosclerosis,AS）形成进程和病变程度.方法 20只C57BL/6背景小鼠,将其中13只种属为C57BL/6背景的ApoE-/-小鼠按随机数字表随机分成两组,实验组7只使用1013个/L的牙周致病菌Pg进行口腔内涂菌接种,持续15周共75次,对照组6只同法口腔内涂擦无菌肉汤培养基,比较两组小鼠体质量、血总胆固醇、三酰甘油及主动脉AS病损面积及病理组织变化的情况.另7只野生型C57BL/6小鼠取其主动脉组织作为正常对照.结果 实验组ApoE-/-小鼠主动脉AS病损平均面积为（98 363.68±12 043.00）μm2,显著大于未接种的小鼠对照组[平均病损面积（62 985.06±7419.64）μm2],P=0.035 两组体质量、血总胆固醇和三酰甘油差异均无统计学意义 与野生型C57BL/6小鼠正常主动脉相比,ApoE-/-小鼠主动脉内壁均有凸向管腔内的AS病损,且动脉壁变平,实验组较对照组AS斑块更明显,动脉管腔更狭窄.结论 Pg口腔内低剂量长期多次接种可以加速ApoE-/-小鼠AS的进展.Objective To assess the effect of longterm and lower oral inoculation with Porphyromonas gingivalis （Pg） on the progression of atherosclerosis in apolipoprotein E-knocked out （ApoE -/-） mice. Methods Six-week-old male ApoE -/- mice were inoculated orally with 0. 1 ml live Pg（1013/L） or bouillon culture-medium quintic per week for 15 consecutive weeks, altogether 75 times of inoculations. The lesion area of atherosclerosis in the aortic tree was measured by en face quantification by red oil O staining method. The atherosclerotic lesion was examined by histopathology. The levels of total cholesterol and triglycerides were compared. Results At 22 weeks after inoculation, the mean atherosclerotic lesion area in inoculated mice was （98 363.68 ± 12 043.00） μm2 ,which was significantly greater than that in noninoculated mice, which was （62 985.06 ± 7419. 64） μm2 （P = 0. 035）.Conclusions Longterm lower oral inoculation of Pg can accelerate the progression of atherosclerosis in apolipoprotein E-knocked out mice.

关 键 词：紫单胞菌 龈 动脉粥样硬化 小鼠 基因敲除 

分 类 号：R543.5[医药卫生—心血管疾病]