周期性牵张力对人牙周膜成纤维细胞Ⅲ型胶原和纤维连接蛋白表达影响的研究  

Effects of cyclic-tension force on expression of collagen-Ⅲ and fibronectin in HPDLF

作　　者：张晓东[1] 宋九余[1] 李永明[2] 林珠[2] 

机构地区：[1]沈阳市沈阳军区总医院口腔内科辽宁省口腔医学研究所,沈阳110840 [2]西安市第四军医大学口腔医学院正畸科,西安710032

出　　处：《口腔医学》2011年第1期16-18,共3页Stomatology

基　　金：辽宁省博士启动基金(20071040)

摘　　要：目的观察周期性牵张力对人牙周膜成纤维细胞(HPDLF)Ⅲ型胶原和纤维连接蛋白表达的影响。方法对培养在弹性膜培养板上的HPDLF施加0.2 Hz、12%形变率的周期性牵张力,利用免疫荧光技术观察Ⅲ型胶原的表达情况;ELISA检测试剂盒检测Ⅲ型胶原和纤维连接蛋白的表达变化。结果对照组HPDLFⅢ型胶原免疫荧光染色弱阳性,实验组加载周期性牵张力后染色明显增强,Ⅲ型胶原合成分泌增加;ELISA检测显示加载周期性牵张力后纤维连接蛋白的表达明显强于对照组。结论周期性牵张力促进HPDLF的Ⅲ型胶原、纤维连接蛋白等细胞外基质的表达。Objective To investigate the effects of the mechanical strain on the expression of collagen-Ⅲ and fibronectin in human periodontal ligament cells（HPDLF）.Methods HPDLF were subjected to 12% elongation by stretching frequency of 0.2Hz in this experiment.The expression of collagen-Ⅲ were observed by immunofluorescence,collagen-Ⅲ and fibronectin by ELISA kit.Results The expression of collagen-Ⅲ in HPDLF was weak positive in control group,while significantly increased after loading cyclic-tension force in experimental group,indicating that the synthesization and secretion of collagen-Ⅲ increased.The expression of fibronectin was significantly increased after loading cyclic-tension force compared with the control group.Conclusions The cyclic-tension force may promote the expression of some ECM such as collagen-Ⅲ and fibronectin.

关 键 词：牵张应力 牙周膜成纤维细胞 胶原蛋白 纤维连接蛋白 

分 类 号：R329.252[医药卫生—人体解剖和组织胚胎学]