L-精氨酸对宫内发育迟缓大鼠肾脏Pax2表达的影响  

Effect of L-arginine on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation

作　　者：盖建芳[1] 谢宗德[1] 李雯[1] 李素萍[1] 胡劲涛[1] 

机构地区：[1]中南大学湘雅二医院新生儿科,湖南长沙410011

出　　处：《中国当代儿科杂志》2011年第2期145-148,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的研究L-精氨酸(L-Arg)对宫内发育迟缓(IUGR)大鼠肾脏Pax2表达的影响。方法孕鼠随机分为3组:正常对照组、IUGR组和干预组。正常对照组母鼠全程以普通饲料(含21%蛋白)喂养。IUGR组和干预组大鼠孕期均采用低蛋白饲料(含10%蛋白)喂养。仔鼠出生后,IUGR组母鼠常规饲料喂养;干预组母鼠在常规饲料喂养的基础上,于部分饮水中每日添加L-Arg 200 mg/kg。取7 d、21 d、2月和3月龄仔鼠肾脏组织,用免疫组化和Western blot法检测Pax2表达。结果免疫组化显示正常对照组仔鼠在各时间点均未见明显Pax2表达。IUGR组、干预组仔鼠2月龄和3月龄时,两组大鼠在肾小球和肾小管区可见Pax2阳性细胞,且3月龄鼠的Pax2表达比2月龄的更显著(P<0.05);干预组的Pax2表达低于同龄IUGR组(P<0.05)。Western blot检测发现,3组仔鼠肾脏在7 d和21 d均未见Pax2蛋白明显表达。到2月龄和3月龄时,干预组和IUGR组仔鼠肾组织Pax2蛋白表达均较正常对照组显著增多,但干预组的表达低于IUGR组(P<0.01)。结论 IUGR大鼠成年后肾脏Pax2基因有再表达现象;用L-Arg干预后Pax2表达明显减少。Objective To study the effect of L-arginine（L-Arg） on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation（IUGR）.Methods Pregnant rats were randomly assigned into three groups：normal,IUGR and L-Arg treated IUGR.The rats in the normal group were fed with ordinary forage（21% protein） during pregnancy.Those in the other two groups were fed with low diet forage（10% protein） during pregnancy.The L-Arg treated group was given drinking water containing L-Arg（200 mg/kg） daily during 21 days of lactation.Pax2 expression in renal tissues was measured with immunohistochemical staining and Western blot in pup rats of 7 days,21 days,2 months and 3 months old.Results The immunohistochemical staining showed that Pax2 was not expressed in the pup rats from the normal group at any time point.Pax2 positive cells were found in renal glomerulus and kidney tubules of 2-months-and 3-months-old rats from the IUGR and L-Arg treated groups.And Pax2 expression in 3-months-old rats was significantly higher than that in 2-months-old rats（P0.05）.L-Arg treatment decreased significantly the Pax2 expression in 2-months-and 3-months-old rats when compared with the untreated IUGR group（P0.05）.Western blot showed that Pax2 protein was not expressed in 7-days-and 21-days-old pup rats from three groups.Pax2 protein expression in 2-months-and 3-months-old pup rats from the IUGR and L-Arg treated groups increased significantly compared with normal controls.Pax2 protein expression in the pup rats from the L-Arg treated group was significantly lower than that in the untreated IUGR pup rats（P0.01）.Conclusions Pax2 is expressed in the kidneys of IUGR rats during adulthood.L-Arg treatment can decrease the expression of Pax2.

关 键 词：宫内发育迟缓 PAX2 肾脏 L-精氨酸 大鼠 

分 类 号：R346[医药卫生—基础医学]