与“全红”瓯江彩鲤体色相关的SRAP及SCAR分子标记  

SRAP AND SCAR MOLECULAR MARKER RELATED TO BODY COLOR OF "WHOLE RED" PATTERNS OF CYPRINUS CARPIO VAR. COLOR

作　　者：吕耀平[1,2] 胡则辉[3] 王成辉[4] 项松平 苏小平 姚子亮 王凯伟 

机构地区：[1]丽水学院化学与生命科学学院,丽水323000 [2]浙江大学动物科学学院,杭州310029 [3]浙江省海洋水产研究所,舟山316100 [4]上海海洋大学农业部水产种质资源与利用重点开放实验室,上海200090 [5]浙江省龙泉市瓯江彩鲤原种场,龙泉323700 [6]浙江省丽水市莲都区水产技术推广站,丽水323000 [7]浙江省丽水市水产技术推广站,丽水323000 [8]浙江省丽水市渔政站,丽水323000

出　　处：《水生生物学报》2011年第1期45-50,共6页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(30700622);浙江省自然基金项目(Y307445)资助

摘　　要：利用相关序列扩增多态性(Sequence Related Amplified Polymorphism,SRAP)技术分析"全红"和"粉玉"瓯江彩鲤,筛选与瓯江彩鲤体色相关的分子遗传标记。从88个SRAP引物组合筛选出的12个引物组合共获得扩增条带104个,并筛选出1个SRAP特异扩增带,即"全红"瓯江彩鲤家系SR2,7173 bp带。该条SRAP特异扩增条带经回收、克隆和测序,并将测序结果进行BLAST分析,发现该片段在GenBank中与斑马鱼的POl多蛋白基因和尿红素基因有较高的同源性。根据序列信息分别设计了4对正、反向引物(22—26 bp)。用4对引物分别在"全红"瓯江彩鲤F2和"粉玉"瓯江彩鲤F2群体中进行PCR扩增,仅发现SC-3(154 bp)能够在"全红"瓯江彩鲤群体中特异扩增,而且在"粉玉"瓯江彩鲤F2群体中未出现此扩增带。采用大样本对该SC-3标记进行验证,结果发现,在"全红"瓯江彩鲤群体中呈现阳性,而在"粉玉"瓯江彩鲤群体中为阴性,可以区分这两种群体。因此SC-3标记可以作为"全红"瓯江彩鲤群体一个重要的分子遗传特征指标,为进一步进行分子标记辅助育种奠定了基础。Oujiang color common carp distributes in Zhejiang Province in southeast part of China,such as Lishui City,Longquan City,Qingtian country and Oujiang river basin.It has several color patterns,including whole red,red with scattered black spots,whole white,white with scattered black spots.Nowadays,several scientific researchers have car-ried out some works relevant to genetic diversity,genetic relationship in Cyprinus carpio var.color.However,we do not find any other studies on color-related molecular markers of Oujiang color common carp.Hence,it is necessary to ob-tain molecular makers for color correlative gene location and marker assisted selection（MAS）.In this paper,Sequence Related Amplified Polymorphism（SRAP） technique was applied to identify the germplasm between ＂whole red＂（＂WR＂） and ＂whole white＂（＂WW＂） population.The specific SRAP fragment,namely SR2,7173bp（SR denote the first two letters of SRAP） from ＂WR＂ and ＂WW＂,was identified from the amplified products of 12 primer pairs screened from 88 SRAP primer pairs.After gel extraction,cloning and sequencing of this specific SRAP band,the sequence was submitted to dbGSS（data of Genome Sequence Survey）.Blast analysis showed that this fragment had high similarity to functional genes of Danio rerio.Four primers（22 to 26 bases） were designed according to the sequence information.Then PCR amplification was carried out between ＂WR＂and ＂WR＂population.The experimental result also indicated that only SCAR-3（abbreviated SC-3,154bp） developed from SR2,7173bp（me2-em7） were ＂WR＂ specificity.Large samples ex-amination suggested that SC-3 could be positively amplified in ＂WR＂ population but not in ＂WW＂ population.Hence,SC-3 could be used a specific molecular marker for germplasm identification,which would provide a powerful,easy and rapid method for discrimination of different populations and genetic analysis.

关 键 词：“全红” “粉玉” 瓯江彩鲤 SRAP标记 SCAR标记 

分 类 号：Q344.5[生物学—遗传学]