中国西藏小反刍兽疫病毒China/Tib/Gei/07-30磷蛋白基因的分子特征及其编码蛋白特性分析  

Sequence Analysis of the Phosphoprotein Gene of Peste Des Petits Ruminants Virus of Chinese Origin

作　　者：包静月[1] 赵文姬[1] 李林[1] 王志亮[1] 吴国珍 吴晓东[1] 刘春菊[1] 王清华[1] 王君玮[1] 刘雨田[1] 李金明[1] 王英丽[1] 

机构地区：[1]中国动物卫生与流行病学中心国家外来动物疫病诊断中心,青岛266032 [2]西藏动物疫病控制中心,拉萨850000

出　　处：《病毒学报》2011年第1期26-33,共8页Chinese Journal of Virology

基　　金：农业部动物疫情监测项目;农业部948项目;公益性行业(农业)科研专项经费

摘　　要：对我国西藏小反刍兽疫病毒野生株China/Tib/Gej/07-30进行磷蛋白基因序列测定,并进行分子生物学特征分析。首先应用逆转录聚合酶链式反应扩增出病毒磷蛋白基因片段,对聚合酶链式反应产物进行直接测序,然后对测定的核苷酸和推测的氨基酸序列进行比较分析。小反刍兽疫病毒China/Tib/Gej/07-30磷蛋白基因由1 655个核苷酸组成,编码2个相互交叠的开放阅读框(ORF)。第一个ORF长度为1 530个核苷酸,编码的P蛋白长度为509个氨基酸。第二个ORF长度为534个核苷酸,编码的C蛋白长度为177个氨基酸。第一个ORF通过基因编辑在751位插入1个G核苷酸,转录生成第二个mRNA,长度为897个核苷酸,编码的V蛋白长度为298个氨基酸。小反刍兽疫病毒China/Tib/Gej/07-30的P蛋白与其他分离株氨基酸序列同源性为86.1%～97.3%,C蛋白氨基酸序列相似性为84.3%～94.9%,V蛋白为82.9%～96.3%。China/Tib/Gej/07-30的P蛋白第315～387位氨基酸是一段高度保守的七肽重复序列。The nucleotide sequences of P gene from a field strain of peste des petits ruminants virus （PPRV） （＂China/Tih/Gej/07-30＂） was firstly determined. The P gene is 1,655 nucleotides long with two overlap- ping open reading frames （ORFs）. The first ORF is 1,530 nucleotides long and would produce P protein of 509 amino acid residues. The second ORF is 534 nucleotides long and would produce C protein of 177 amino acid residues. The first ORF produces a second mRNA transcript of 897 nucleotides long with an extra G nucleotide at position 751. Translation from this mRNA would produce V protein of 298 amino acid residues. The nucleotide and deduced amino acid sequence were compared with the homologous region of other PPRV isolates. At the amino acid level, the ＂China/Tib/Gej/07-30＂ shares homology of 86.1%-97.3%, 84.3%-94.9%, and 82.9%-96.3% for P, C, and genes were identified on the basis of conservation V proteins respectively. Several sequence motifs in the P in the PPRVs and the morbilliviruses.

关 键 词：小反刍兽疫病毒 磷蛋白 C蛋白 V蛋白 核苷酸序列 基因编辑 

分 类 号：S852.659.5[农业科学—基础兽医学]