融合表达DEC 205单链抗体和NS3蛋白的HCV DNA疫苗的免疫原性研究  

Enhancement of the Immune Response in Mice with a Noval HCV DNA Vaccine Targeting NS3 to Dendritic Cells

作　　者：殷霄[1] 王文[1] 谭文杰[1] 邓瑶[1] 管洁[1] 文波[1] 陈红[1] 阮力[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒病应急技术中心,北京100052

出　　处：《病毒学报》2011年第1期44-49,共6页Chinese Journal of Virology

基　　金：国家863课题2007AA02Z455

摘　　要：为研发新型HCV DNA疫苗并探讨优化其免疫原性的策略,我们分析靶向树突状细胞(Dendritic cells,DC)的分子对HCV DNA疫苗免疫原性的影响。我们基于抗小鼠DC细胞表面分子DEC205/CD205的单克隆抗体DEC205的单链分子,构建可单独表达DEC205单链抗体或者与HCV非结构蛋白NS3融合表达的DNA表达质粒,并构建单独表达HCV非结构蛋白NS3的DNA表达质粒;经瞬时转染法鉴定HCV NS3及其与DEC205单链抗体融合蛋白的表达;随后采用注射结合电转的方式免疫Balb/C小鼠并研究各疫苗的体液(NS3特异性IgG抗体)与细胞免疫(IFN-γELISPOT)效果。结果表明:DEC205单链抗体基因与HCV NS3编码基因的融合可显著增强NS3特异的免疫应答;采用皮内注射加卡钳电极电转的方式可以产生最强的NS3特异性抗体和T细胞免疫反应。因此,通过DEC205单链抗体与HCV DNA疫苗靶抗原融合可明显增强免疫应答效果。该策略为HCV及其他类似病原的新型DNA疫苗研制提供重要依据。To develop a new Hepatitis C virus （HCV） DNA vaccine ,We explored strategies for optimizing the immunogenicity of HCV DNA vaccine in fusion with dendritic cell-targeting molecules （scDEC, a sin- gle-chain antibody against the murine DC cell surface molecule DEC205）. We constructed the DNA vaccine ptasmids expressing the HCV non-structural protein NS3 alone or in combination with DEC205 as a fusion protein and identified the expression of the molecules of interest by transient transfection of 293 cells with the resultant DNA vaccine plasmids. Then BALB / C mice were immunized with these plasmids by the in jection in combination with electroporation. The NS3-specific IgG antibody（ELISA） and cellular immunity （IFN-γ ELISPOT） were analyzed post twice immunization. Our results showed that., the single-chain antibody against DEC205 fused with vaccine antigen NS3 significantly enhanced the immunogenicity of new HCV DNA vaccine, the intradermal injection in combination with electroporation using caliper electrodes resulted in most robust NS3-specific antibody and T cell immune response. In conclusion,immune response for the HCV NS3 protein-encoding DNA vaccine was enhanced significantly when targeting antigen NS3 to DCs by scDEC. The present strategy could merit further study in the context of other prophylactic and therapeutic DNA based vaccines.

关 键 词：丙型肝炎病毒 DNA疫苗 NS3抗原 小鼠DEC205单链抗体 

分 类 号：Q78[生物学—分子生物学]