北柴胡不定根培养及茉莉酸甲酯处理对柴胡皂苷含量的影响  

Cultivation of Adventitious Roots and Effect of Methyl Jasmonate on its Saikosaponins Contents for Bupleurum chinense DC.

作　　者：战晴晴[1,2,3] 金钺[1] 魏建和[1] 张杰[3] 隋春[1] 

机构地区：[1]中国医学科学院/北京协和医学院药用植物研究所,北京100193 [2]中国医学科学院/北京协和医学院药用植物研究所海南分所,海南万宁571533 [3]东北林业大学生命科学院,黑龙江哈尔滨150040

出　　处：《生物技术通讯》2011年第1期57-60,共4页Letters in Biotechnology

基　　金：国家科技支撑计划重点项目(2006BA109B01);北京市自然科学基金(5102033)

摘　　要：目的:建立北柴胡不定根培养体系,明确茉莉酸甲酯(MeJA)处理对北柴胡不定根中柴胡皂苷含量积累的影响。方法:利用固体和液体相结合的组织培养技术培养北柴胡不定根;分别以不同浓度的MeJA处理不定根不同时间,利用HPLC测定处理后不定根中柴胡皂苷含量的积累变化。结果:培养了北柴胡不定根;MeJA处理对北柴胡不定根中柴胡皂苷含量的积累有明显促进作用,当MeJA浓度为200μmol/L时,柴胡皂苷含量最高,为0.45%;以200μmol/L MeJA处理北柴胡不定根26 d时,柴胡皂苷含量最高,为0.51%。结论:北柴胡不定根培养结合MeJA诱导,可做为柴胡皂苷次生代谢合成途径及其积累规律研究的有效技术体系。Objective：To cultivate adventitious roots of Bupleurum chinense DC.and to assay the effect of methyl jasmonate（MeJA） on saikosaponins contents in B.chinense adventitious roots.Methods：Using solid medium and liquid medium to cultivate B.chinense adventitious roots,and HPLC was used to measure the saikosaponins content in control and MeJA-treated adventitious roots.Results：In this study,B.chinense adventitious roots were successfully cultivated and it was clear that MeJA remarkably promoted its saikosaponins biosynthesis.When 200 μmol/L MeJA was added to the media,the saikosaponins content of adventitious roots reached 0.45%;and when adventitious roots were cultivated lasting for 26 days with 200 μmol/L MeJA added to the media,its saikosaponins content reached 0.51%.Conclusion：Cultivation of B.chinense adventitious roots adding with MeJA induction was a potent research tool which can be used in studies on saikosaponins biosynthesis pathway and saikosaponins accumulation features

关 键 词：北柴胡 不定根 柴胡皂苷 茉莉酸甲酯 

分 类 号：Q942[生物学—植物学]