微透析法观察蜕皮甾酮对家兔蛛网膜下腔出血的作用  

Effect of ecdysterone on subarachnoid hemorrhage in rabbit:a microdialysis study

作　　者：刘智[1] 陈渝杰[1] 陈志[1] 唐卫华[1] 朱刚[1] 王宪荣[1] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科,重庆400038

出　　处：《中华神经外科疾病研究杂志》2011年第1期41-44,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：国家十一五科技支撑计划基金资助项目(2006BAI01A12);自然科学基金资助项目(30500662;30772224;30801186);军队十一五中医药攻关课题基金资助项目(2006181002)

摘　　要：目的通过微透析的方法,观察蛛网膜下腔出血(SAH)后家兔海马内能量物质和谷氨酸的含量变化,了解蜕皮甾酮(EDS)对其干预的效果。方法 30只家兔分为5组,即结扎右侧颈总动脉组(L组)、结扎后枕大池二次注血组(L-SAH组)、结扎后枕大池二次注血尼莫地平治疗组(Nim/SAH组)、结扎后枕大池二次注血蜕皮甾酮(1 mg/kg,4/d)治疗组(EDS/SAH 1 mg组)、结扎后枕大池二次注血蜕皮甾酮(3 mg/kg,4/d)治疗组(EDS/SAH 3 mg组)。比较观察微透析所测得的各组家兔海马SAH前后葡萄糖、丙酮酸、乳酸、谷氨酸的含量变化。结果乳酸/丙酮酸比值(L/P)在SAH后有上升趋势。SAH后3 d时EDS/SAH 3 mg组右侧海马组织间液中L/P较其它组低(P<0.05)。谷氨酸的含量在SAH后也明显增高,10 d时达高峰。Nim/SAH组1 d和3 d右侧海马组织间液谷氨酸含量较L-SAH组降低(P<0.05),在SAH后1 d和3 d EDS/SAH 3 mg组较L-SAH组和Nim/SAH组也明显降低(P<0.01)。结论蜕皮甾酮能减轻家兔SAH后海马组织能量代谢的紊乱和谷氨酸的堆积。Objective To investigate the effect of ecdysterone on metabolism and glutamate concentration changes of hippocampus in rabbit following subarachnoid hemorrhage（SAH） by microdialysis method.Methods A total of 30 rabbits were divided into 5 groups randomly：L group（ligation of the right common carotid artery）,L-SAH group（injection of autologus arterial blood into the cistern magana twice 7 d after ligation of the right common carotid artery）,Nim/SAH group（nimodipine treatment after SAH）,EDS/SAH 1 mg group（1 mg/kg ecdysterone treatment 4 times per day after SAH） and EDS/SAH 3 mg group（3 mg/kg/d EDS treatment 4 times per day after SAH）.The concentrations of glucose,lactate,pyruvate,and glutamate in hippocampus before and after SAH in all rabbits were measured by microdialysis system.Results The lactate and pyruvate ratio（L/P） was increased after SAH,and L/P of the right hippocampus in EDS/SAH 1 mg group was lower than that of the other groups at 3 d after SAH（P0.05）.Glutamate concentration was increased after SAH,peaking at 10 d.Glutamate concentration of the right hippocampus in Nim/SAH group was lower than that of L-SAH group at 1 d and 3 d after SAH（P0.05）,and glutamate concentration in EDS/SAH 3mg group was lower than that of L-SAH and Nim/SAH groups（P0.01）.Conclusion EDS can alleviate the metabolism and glutamate disorders of hippocampus after SAH in rabbit.

关 键 词：蛛网膜下腔出血 微透析 蜕皮甾酮 家兔 

分 类 号：R965.1[医药卫生—药理学]