IN-1和NT-3联合作用对大鼠脊髓组织c—fos和c—jun基因表达变化的影响  

The change of expression of c-fos and c-jun gene when using IN-1 alone or combination with NT-3 after spinal cord injury

作　　者：詹瑞森[1] 孙双喜[1] 王卫国[1] 陈世杰[1] 

机构地区：[1]中南大学湘雅三医院骨科,长沙410013

出　　处：《中国医师杂志》2011年第1期9-11,15,共4页Journal of Chinese Physician

基　　金：湖南省卫生厅项目（B2005-076）

摘　　要：目的探讨脊髓损伤后给予IN-1以及和NT-3联合作用后c—fos及c-jun的表达有无变化。方法健康Sprague—Dawley（SD）大鼠120只，随机分为损伤对照组、IN-1组及IN-1和NT-3联合作用组。各组分别在相应时间点取材行RT-PCR检测c—fos和c-jun基因表达的变化。结果IN-1作用后c—fos、c-jun表达分别出现下降和升高，联合应用NT-3后更为明显，其中c—fos的峰值手术对照组为0．974±0．126、IN-1组为0．834±0．047、联合作用组为0．698±0．052，c—jun的峰值手术对照组为0．642±0．048、IN-1组为0．712±0．050、联合作用组为0．814±0．041。结论NT-3、IN-1作用机制之一可能通过抑制c—fos表达，促进c-jun表达对脊髓损伤起保护作用。Objective To study the change of expression of c-fos and c-jun when using IN-1 alone or combination with NT-3 after spinal cord injury. Methods 120 adult health Sprague-Dawley （SD） rats were random divided into three groups, including control group, IN-1 group, and IN-1 combination with NT-3 group. All rats were killed at the scheduled time and its myeloid tissues were taken out. In each group, the expression of c-fos and c-jun gene was detected by using reverse transcription- polymerase chain reaction technique （RT-PCR）. Result The transcriptional levels of c-fos decreased and .c-jun increased when using IN-1 alone, and the levels changed more when using IN-1 combination with NT-3. The peak of c-los reached to 0. 974 ±0. 126 in control group, 0. 834 ±0. 047 in IN-1 group, and 0. 698 ±0. 052 in IN-1 combination with NT-3 group, and the peak of c-jun reached 0. 642 ±0. 048, 0. 712 ±0. 050, and 0. 814 ± 0. 041, respectively. Conclusion One of the mechanisms of IN-1 and NT-3 to protect the spinal cord might be through inhibiting the expression of c-fos and enhancing the expression of c-jun.

关 键 词：脊髓损伤/药物疗法 神经营养因子3/投药和剂量 神经生长因子类/拮抗剂和抑 制剂/投药和剂量 抗体 单克隆/投药和剂量 脊髓/药物作用/代谢 原癌基因蛋白质c—fos/代谢 原 癌基因蛋白质c-jun/代谢 

分 类 号：R651.2[医药卫生—外科学]