胡子鲶血浆中外源生长激素的代谢动力学  

PLASMA KINETICS OF RECOMBINANT COMMON CARP GROWTH HORMONE IN CLARIAS BATRACHUS

作　　者：肖东[1] 陈松林[2] 严安生[3] 陈细华[2] 邓文涛[2] 

机构地区：[1]中山大学生命科学学院,广州510275 [2]中国水产科学研究院长江水产研究所,沙市434000 [3]华中农业大学水产学院,武汉430070

出　　处：《水产学报》1999年第3期234-240,共7页Journal of Fisheries of China

基　　金：农业部九五重点科研资助

摘　　要：采用草鱼生长激素夹心式酶联免疫吸附测定法（ＥＬＩＳＡ） ，研究了重组鲤生长激素（ｒｃＧＨ） 在胡子鲶血浆中的代谢动力学。肌肉注射ｒｃＧＨ（０．２ 和ｌ μｇｇ） 后， 胡子鲶血浆ｒｃＧＨ 水平迅速升高， 于２ 和３ｈ 时达峰值， 随后下降，在注射后第２ 和４ 天， 血浆中检测不到ｒｃＧＨ。腹腔注射ｒｃＧＨ（０ ．２ 和ｌμｇｇ） 后， 胡子鲶血浆ｒｃＧＨ水平也有同上类似的变动规律。胡子鲶腹腔注射０ ．５、１ 和２ μｇｇ 的ｒｃＧＨ，７ 天后各组鱼血浆中的ｒｃＧＨ 均被清除干净。向尾部血管内注射ｒｃＧＨ（０ ．２ 和０．５ μｇｇ） 后，胡子鲶血浆ｒｃＧＨ 水平下降遵循指数性衰减规律，２４ｈ 后血浆中ｒｃＧＨ 均未清除干净。Using the sandwich enzyme linked immunosorbent assay(ELISA) for grass carp growth hormone , plasma kinetics of rcGH in Clarias batrachus after administrations of rcGH were investigated. After intramuscular injections of rcGH (0.2 and l μg/g) into C.batrachus,the plasma rcGH levels were increased, peak values were seen at the end of 2 and 3 hours post injection,and rcGH in plasma was undetected on the 2nd and 4th days post injection, respectively. Similar uptake and clearance patterns were seen after intraperitoneal injections rcGH(0.2 and l μg/g) into C.batrachus . Intraperitoneal injections of rcGH (0.5,1 and 2 μg/g) to walking catfish, rcGH in plasma was all undetected on the 7th day post injection. When rcGH( 0.2 and 0.5 μg/g) was injected into intra caudal vessel of C.batrachus , plasma rcGH levels decreased by way of exponential decay,rcGH in plasma was detectable 24h postintra caudal vessel injection.

关 键 词：胡子鲶 重组鲤生长激素 血浆代谢动力学 

分 类 号：Q959.468[生物学—动物学] S965.128[农业科学—水产养殖]