血管平滑肌细胞的体外氧化应激反应及雌激素的影响  

RESPONSES OF VASCULAR SMOOTH MUSCLE CELLS TO OXIDATIVE STRESS IN VITRO AND THE EFFECTS OF ESTROGEN

作　　者：徐菁[1] 朱从丽[2] 王乔[2] 宋健[2] 

机构地区：[1]南华大学医学院人体解剖学教研室,湖南衡阳421001 [2]武汉大学医学院人体解剖学与组织胚胎学系医学研究中心,湖北武汉430071

出　　处：《中国组织化学与细胞化学杂志》2010年第6期537-541,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(30470906);中澳科技合作特别基金(30711120185)

摘　　要：目的探讨雌激素对VSMC氧化应激反应的影响及机制。方法第3-4代雌性大鼠血管平滑肌细胞(VSMC)在有或无10-8mol/L 17b-雌二醇(E2)存在下用不同浓度(0-250mmol/L)H2O2诱导氧化应激;MTT法,流式细胞术,Western blot分别检测VSMC活力,细胞周期,MAPK信号通路活性的变化。结果当浓度低于25mmol/L时,H2O2对VSMC活力无影响;当浓度为50-150mmol/L时,VSMC活力呈剂量依赖性下降,并伴随G0/G1期细胞升高;当浓度达到200mmol/L时,细胞活力下降至最低点并出现凋亡峰。10-8mol/L E2可显著抑制150mmol/L H2O2诱导的VSMCERK和p38的磷酸化、从而缓解VSMC G0/G1期阻滞,并降低高浓度H2O2诱导的VSMC凋亡。结论中等浓度的H2O2(50-150mmol/L)抑制VSMC增殖;高浓度H2O2(≥200mmol/L)不仅抑制VSMC生长、且导致部分VSMC凋亡;雌激素可通过抑制ERK和p38活性对氧化应激的VSMC起保护作用。Objective To investigate the effects of estrogen on the responses of vascular smooth muscle cells（VSMCs） to oxidative stress.Methods The sub-cultured（passage 3-4） female rat VSMCs were treated with different concentrations（10-250mol/L） of H2O2 in the presence or absence of 10-8mol/L E2.The viability,cell cycle progress and MAPK signaling activity were analysed with MTT assay,flow cytometry and Western blot,respectively.Results When the VSMCs were treated with 10-25mol/L H2O2,the cellular viability had no significant changes,whereas of 50-150mol/L H2O2 led to a dose-dependent decrease in cellular viability and an obvious G0/G1 cell cycle arrest.When the concentration of H2O2 reached 200mol/L,the viability of VSMCs descended to the lowest level with appearance of an obvious sub-G0/G1 population.In the preasence of 10-8mol/L E2,all the changes as well as the H2O2-induced ERK and p38 phosphorylation were significantly mitigated.Conclusion In vitro,50-150mol/L H2O2 inhibits VSMC growth,and high levels（≥200mol/L） of H2O2 cause both growth arrest and apoptosis.Through inhibition of the H2O2-induced activities of ERK and p38,estrogen shows a protective effect on VSMCs undergoing oxidative stress.

关 键 词：雌激素 血管平滑肌细胞 氧化应激 过氧化氢 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]