山萘杀线虫活性成分的分离及鉴定  

Isolation and Identification of Nematicide Component from Kaempferia galanga L.

作　　者：张天柱[1] 曾勇[1] 朱福伟[1] 胡美英[1] 翁群芳[1] 

机构地区：[1]华南农业大学昆虫毒理研究室天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《西北植物学报》2010年第12期2524-2529,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30400037)

摘　　要：采用超声波提取法,用甲醇作溶剂对山萘进行粗提,分别测定了山萘提取物对松材线虫和南方根结线虫的毒性,结果显示,1 000 mg/L山萘甲醇粗提取物处理松材线虫和南方根结线虫,24 h校正死亡率均达到100%。采用活性追踪和柱层析法,以5种植物病原线虫为对象,提取、分离、纯化出山萘根中的活性成分,结果显示,在正己烷萃取组分中,得到活性较高的化合物A、B;用化合物A处理南方根结线虫、松材线虫、马铃薯腐烂茎线虫、海南根结线虫、象耳豆根结线虫72 h后,其LC50分别为17.79、29.70、43.21、57.64和36.94 mg/L;用化合物B处理72 h后LC50分别为1.49、2.81、10.09、26.67和14.47 mg/L。化合物A和B经1H-NMR1、3C-NMR、GC-MS分析鉴定表明,A为肉桂酸乙酯,B为对甲氧基肉桂酸乙酯。Kaempferia galanga L.was extracted by ultrasonic method using methanol as the solvent.And their toxicity of the extracts against Bursaphelenchus xylophilus and Meloidotyne incongnita were tested.The results showed that the mortality of extracts from K.galanga L against B.xylophilus and M.incongnita with 100% mortality at 1 000 mg/L after 24 h.Antifungal components were extracted,isolated and identified from K.galanga L by bioassay-guided and silica gel column chromatography.Compound A and B with excellent nematicide activity had been obtained from petroleum hexane extracts.After treatment 72 h,the LC50 of component A against M.incongnita,B.xylophilus,Ditylenchus destructor,M.eloidogyne hainensis,M.enterolobii were 17.79,29.70,43.21,57.64 and 36.94 mg/L,respectively.The LC50 of component B against M.incongnita,B.xylophilus,D.destructor,M.eloidogyne hainensis,M.enterolobii were 1.49,2.81,10.09,26.67 and 14.47 mg/L.Compound A was identified by 1H-NMR,13C-NMR and GC-MS as ethyl cinnamate,Compound B is ethyl P-Methoxyl cinnamate.

关 键 词：山萘 线虫 分离 鉴定 肉桂酸乙酯 对甲氧基肉桂酸乙酯 

分 类 号：Q501[生物学—生物化学]