浸矿细菌胞外聚合层中多糖含量的测定  

Determination of polysaccharide content in extracellular polymers of leaching bacteria

作　　者：富瑶[1] 杨洪英[1] 范有静[1] 张长征[1] 

机构地区：[1]东北大学材料与冶金学院,辽宁沈阳110819

出　　处：《中南大学学报（自然科学版）》2010年第5期1686-1690,共5页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(50674029;50874030);国家高技术研究发展计划项目(2006AA06Z127);高等学校博士学科点专项科研基金资助项目(20060145015);中央党校基本科研业务费专项资金资助项目(N090602010)

摘　　要：以稳定期的浸矿细菌菌液为研究对象,探讨苯酚-硫酸法测定浸矿细菌胞外聚合层中多糖含量的最佳条件,并用红外光谱检测浸矿细菌胞外聚合层中多糖的结构组成。研究结果表明,苯酚-硫酸法测定浸矿细菌胞外聚合层中多糖含量的最佳条件为:最大吸收波长490 nm,浓硫酸用量5 mL,苯酚溶液质量分数5%;在最佳条件下测得的浸矿细菌胞外多聚合层中的多糖质量浓度为12 mg/L;由红外光谱分析浸矿细菌胞外聚合层中的多糖,发现其结构中含有O—H,C=C,—CH3和C—H键。The leaching bacteria liquid in the stationary phase was studied.The optimum condition was discussed for the polysaccharide content determined by phenol-sulfuric acid method in the extracellular polymers of leaching bacteria.The infrared spectrum was used to detect the structure of the polysaccharide in the extracellular polymers of leaching bacteria.The results show that the optimum conditions are 490 nm for the maximum adsorption wavelength,5 mL of H2SO4 and 5% phenol（mass fraction）.The measured value is 12 mg/L for the polysaccharide content in the extracellular polymers of leaching bacteria by phenol-sulfuric acid method in the optimum condition.IR analysis results show that the O—H,C=C,—CH3 and C—H bond exist in the extracellular polymers of leaching bacteria.

关 键 词：苯酚-硫酸法 浸矿细菌 细菌胞外聚合层 多糖 红外光谱 

分 类 号：TF80[冶金工程—有色金属冶金]