维拉帕米和普奈洛尔钾停搏液保护幼大鼠心脏功能的研究  

Effect of Kalium-Verapamil-Propranolol Cardioplegia on Function of Immature Rat Heart

作　　者：方秋娟[1] 王雅锋[1] 王瑞幸[1] 吴枝娟[1] 何瑞岚[1] 叶恩典[1] 

机构地区：[1]福建医科大学基础医学院生理学与病理生理学系,福州350004

出　　处：《福建医科大学学报》2010年第6期421-425,共5页Journal of Fujian Medical University

基　　金：福建省教育厅科技重点项目(JA07081)

摘　　要：目的观察含维拉帕米、普奈洛尔的钾("钾维普")停搏90 min对幼大鼠心脏功能影响,并与缺血、高钾液停搏及连续灌流不停搏心脏比较。方法离体幼大鼠心脏Langendorff法灌流20 min,分4组(n=8):正常灌注组(CON),连续灌流150 min;缺血-再灌注组(I/R),无糖不充氧修正洛氏液灌3 min停灌27 min连续3阵(缺血90 min)再灌注60 min;高钾停搏液保护组(ST.T)和"钾维普"停搏液保护组(KVP),缺血期间每次3 min灌注分别采用ST.ThomasⅡ停搏液、含维拉帕米6.8×10-7mol/L和普奈洛尔1.1×10-7mol/L的ST.ThomasⅡ停搏液。实验全程170 min维持37℃,实时监测心率、心肌张力、收缩力、最大收缩速度、最大舒张速度及冠脉流量等参数。结果 CON组幼大鼠心脏离体灌流150 min,心率、收缩力、冠脉流量呈下降性变化,心肌张力无明显改变;I/R组缺血90 min期间心肌张力升高,复灌后呈紊乱的颤动未能复搏;与CON组比较,ST.T组缺血60 min后心肌张力升高明显(P<0.05),复灌后收缩力小(P<0.05);与ST.T组比较,KVP组缺血90 min期间心脏张力低(P<0.05),复灌后心搏各参数恢复好(P<0.05),冠脉流量高(P<0.05),其中心率、收缩力、收缩舒张速度、冠脉流量均高于CON组(P<0.05)。结论 "钾维普"停搏保护的未成熟心脏功能优于连续灌流不停搏和缺血、高钾液停搏的心脏功能。Objective To observe the protective effect of Kalium-Verapamil-Propranolol（KVP） Cardioplegia on the function of immature rat myocardium.Methods Thirty-two hearts isolated from Sprague-Dawley rats of 60～80 g body weight,aged（22±2） days,male or female,were perfused with Langendorff method for 20 min and assigned to 1 of the following 4 groups（n=8）： control（CON group）,which was continuously perfused for 150 min;ischemia/reperfusion（I/R group）,which received 3 cycles of a perfusion with Locke＇s solution without glucose and oxygen for 3 min followed by no perfusion for 27 min,and then a reperfusion for 60 min;ST.T group and KVP group were respectively perfused with ST.Thomas II cardioplegia and KVP cardioplegia（ST.Thomas II cardioplegia containing Verapamil 6.8×10-7mol/L and Propranolol 1.1×10-7mol/L） during 3 cycles ischemia.Cardiac function indexes,including heart rate,Tension,contraction force,peak systolic velocity,peak diastolic velocity,coronary flow,and re-beat time,were monitored during the ischemic-reperfusion.Results In CON group,the function of the isolated immature heart decreased after 150 min perfusion;In I/R group,cardiac tension was elevated after 90 min ischemia,and hearts failed to beat again after reperfusion;In ST.T group,cardiac tension was also elevated after 90 min ischemia,and the heart beating recovered slightly after reperfusion.Compared with ST.T group,KVP group showed decreased cardiac tension（P0.05） and best recovery of heart beating after reperfusion（P0.05）,which is better than that of continuous perfusion.Conclusion KVP cardioplegia possesses better protective effects on the function of the immature heart than the continuous perfusion and ST.Thomas II cardioplegia.

关 键 词：心麻痹液 钾 心肌再灌注损伤 心脏 维拉帕米 普奈洛尔 疾病模型 动物 

分 类 号：R965[医药卫生—药理学]