氯化钴对急性下肢缺血大鼠模型缺氧诱导因子1α表达的影响  

Effect of CoCl_2 on Hypoxia-Inducible Factor-1α Expression in an Acute Hindlimb Ischemia Rat Model

作　　者：李南[1] 陈幸生[1] 官云彪[1] 林梃[1] 

机构地区：[1]福建医科大学附属协和医院普外科,福州350001

出　　处：《福建医科大学学报》2010年第6期426-428,435,共4页Journal of Fujian Medical University

基　　金：福建省卫生厅青年科研基金(2007-1-21)

摘　　要：目的观察氯化钴对急性下肢缺血模型缺氧诱导因子-1α(HIF-1α)表达的影响,探讨氯化钴治疗肢体动脉闭塞症的可能性。方法 20只雄性SD大鼠,随机均分成4组:手术+氯化钴、手术+生理盐水、假手术+氯化钴、假手术+生理盐水。手术行左股动脉结扎,术后1 d腹腔注射氯化钴(60 mg/kg),或等容积生理盐水。各组术前1 d、术后1 d(腹腔注射前)、2、4、6 d左腓肠肌穿刺活检。免疫组织化学方法检测HIF-1α的表达。结果股动脉结扎手术可增加术侧肢体骨骼肌HIF-1α的表达(t=5.963,P=0.000),缺血肢体经氯化钴处理后HIF-1α的表达明显高于对照组(F=18.074,P<0.05)。结论氯化钴可以增加缺血肢体骨骼肌的HIF-1α的表达,可望用于治疗肢体动脉闭塞症。Objective To study the effect of CoCl2 on Hypoxia-Inducible Factor-1α（HIF-1α） expression in a hindlimb ischemia rat model.Methods 20 male SD rats were randomly divided into 4 groups（n=5）：operation＋ CoCl2 group,operation＋NS group,sham operation＋ CoCl2 group and sham operation＋ NS group.Hindlimb ischemia rat model was established by ligating femoral artery.On post operative 1d,all rats received intraperitoneal injection of CoCl2（60 mg/kg） or NS as placebo.The expression of HIF-1α in different groups was detected 1 day before operation and on post operative 1,2,4 d and 6 d respectively by immunohistochemistry.Results The HIF-1α expression in operation groups was higher than that in sham operation groups（t=5.963,P=0.000）.And the HIF-1α expression in operation＋ CoCl2 groups was also higher than that in operation＋NS groups（F=18.074,P0.05）.Conclusions Intraperitoneal injection of CoCl2 can increase HIF-1α expression in ischemia limbs.

关 键 词：钴 缺氧诱导因子1 Α亚基 动脉闭塞性疾病 股动脉 缺血 下肢 缺氧 疾病模型 动物 

分 类 号：R543.5[医药卫生—心血管疾病] R-332[医药卫生—内科学]