禽脑脊髓炎病毒在BGM-70传代细胞系上培养特性的初步研究  

A Preliminary Study on the Culture Characteristics of Avian Encepholomyelitis Virus in Continuous Cell Line BGM-70

作　　者：刘爵[1] 张杰[1] 刘有昌[1] 张方亮[1] 周蛟[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100089

出　　处：《华北农学报》1999年第3期136-140,共5页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金

摘　　要：进行了 禽脑脊 髓炎病 毒 Ｌ２ Ｚ 株在 Ｂ Ｇ Ｍ７０ 传 代 细胞 系上 培 养特 性的 研 究。该 毒 株 在细胞上盲 传３ 代后 能产生明 显的细 胞病变，并 且传代 过程中 细胞 病变 稳定 地出 现。利 用琼 脂扩 散试验、免疫 荧光试 验、免疫酶 试验及 负染电镜 观察 研究 表 明， Ｌ２ Ｚ 株 接种 Ｂ Ｇ Ｍ７０ 细 胞具 有良 好 的增殖特性 ，并且病 毒是在细 胞浆中 进行复制 。该研究 报道禽 脑脊髓 炎病 毒能 成功 地在 传代 细胞 系上培养增 殖。A study on the culture characteristics of avian encephalomyelitis virus (AEV)strain L 2Z in continuous cell line BGM 70 was carried out.The strain blindly passaged to the third generation in BGM 70 cell line caused pronounced cytopathic effects (CPE),and produced consistently CPE from the third passage.These results from the agar gel diffusion precipitin(AGP),indirect immunofluorescence antibody(IFA),indirect immunoenzyme assay (IIEA)and negatively stained electron microscope(NSEM) techniques showed that the strain L 2Z adapted to BGM 70 cell line had good multiplication characteristics,and its replicated area was cytoplasma.This paper is the first report on the successful cultivation and multiplication of avian encephalomyelitis virus in continuous cell line.

关 键 词：脑脊髓炎病毒 BGM-70 传代细胞系 培养特性 

分 类 号：S858.305.3[农业科学—临床兽医学]