原花青素对矽尘致大鼠肺组织转化生长因子-β_1和热休克蛋白47表达的抑制作用  

Depression effect of proanthocyanidins on expression of pulmonary TGF-β_1 and HSP47 in silica exposed rats

作　　者：曹福源[1,2] 闫立成[2] 李清钊[2] 张艳淑[2] 王广增[2] 姚林[1,2] 

机构地区：[1]华北煤炭医学院医学实验动物中心 [2]华北煤炭医学院预防医学系河北省煤矿卫生与安全实验室,河北唐山063000

出　　处：《中国工业医学杂志》2011年第1期6-8,F0003,共4页Chinese Journal of Industrial Medicine

摘　　要：目的探讨原花青素(proanthocyanidins,PC)对染尘大鼠肺纤维化进程的具体作用。方法 SPF级SD大鼠96只,随机分为对照组、矽肺组、PC低剂量组、PC高剂量组,每组24只;采用气管内注射SiO2悬液方法复制大鼠矽肺模型。染尘后第2天PC低剂量组按50 mg/(kg.d)灌胃给予PC,PC高剂量组按500 mg/(kg.d)灌胃给予PC,矽肺组和对照组则给予等容生理盐水。给药开始后第14、28、60天时每组随机处死8只大鼠;应用免疫组化法观察肺组织TGF-β1、HSP47的表达,并检测大鼠血清超氧化物歧化酶(SOD)活力。结果与对照组比较,大鼠在染尘14、28和60 d时,血清中SOD的活力明显下降,60 d时尤为显著;此外,肺组织中TGF-β1和HSP47的表达增加,且随染尘时间增加呈现上升趋势。应用高剂量PC干预后,血清中SOD活性增加(P<0.05),在染尘60 d时,上升到251.36 U/ml(矽肺组为181.32 U/ml);PC干预组肺组织中TGF-β1和HSP47表达的升高趋势(P<0.05)亦见恢复,染尘60 d时,PC高剂量组肺组织中TGF-β1和HSP47的表达分别为24.45和13.54(矽肺组为33.27和18.70)。结论原花青素可增强SOD活力,减少TGF-β1和HSP47的表达,提示可在一定程度上缓解染尘大鼠肺纤维化进程。Objective To explore the effect of proanthocyanidins（PC）on the pulmonary fibrosis in silica-exposed rats.Methods Ninety-six SD rats were randomly divided into four groups：control group,silicosis group,low dose and high dose PC groups,24 rats in each group.Silica（50 mg/ml）was given by direct tracheal instillation;PC groups were administered with PC by gastric lavage at dose of 50 mg/（kg·d）（low dose group）and 500 mg/（kg·d）（high dose group）respectively at the second day of exposure to silica,rats in control and silicosis group were administered with saline solution at the same time.Eight rats for each group were killed at the 14,28 and 60 days after silica instillation,take the blood and lung tissue samples rapidly.The expressions of TGF-β1 and HSP47 in lung tissues were detected by immunohistochemical analysis,the activity of SOD in serum was also measured.Results Compared with that of control group,activity of SOD was decreased significantly at 28th and 60th days after silica exposure,the expressions of TGF-β1 and HSP47 were increased significantly with the time;while activity of SOD in high dose PC group was increased significantly（P0.05）,and expressions of TGF-β1 and HSP47 in both low and high dose PC groups were decreased significantly（P0.05）compared with that in silica exposed group at the 60th day after silica exposure.Conclusion PC administration could increase activity of serum SOD and reduce expression of TGF-β1 and HSP47 in lung,which suggested that PC might have the effect of relieving pulmonary fibrosis caused by silica.

关 键 词：原花青素 组织转化生长因子β1(TGF-β1) 热休克蛋白47(HSP47) 肺纤维化 矽肺 

分 类 号：R135.2[医药卫生—劳动卫生]