非生物胁迫对盐生杜氏藻DsMPK转录、翻译和磷酸化修饰的影响  

Effect of Abiotic Stress on Transcription, Translation and Phosphorylation of DsMPK in Halotolerant Algae Dunaliella salina

作　　者：雷高鹏[1] 冯晓虎 徐辉[1] 孙建瑞[1] 谢力[1] 曹毅[1] 

机构地区：[1]四川大学生命科学学院微生物与代谢工程四川省重点实验室,成都610064 [2]成都生物制品研究所,成都610023

出　　处：《应用与环境生物学报》2011年第1期29-33,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(Nos.30871321;30971817)资助~~

摘　　要：促有丝分裂活化蛋白激酶(Mitogen-activated protein kinase,MAPK或MPK)途径是在真核生物中广泛存在的调控多种生理过程的信号途径,在细胞增殖、分化、凋亡、生物和非生物胁迫等生理过程的调控中起着关键的作用.盐生杜氏藻(Dunaliella salina,盐藻)是目前世界上最耐盐的真核光合生物之一.在前期实验中,已发现盐藻MAPK(DsMPK)的表达受到低温和高盐胁迫的抑制.分析DsMPK在高温、低盐、氧化、紫外胁迫中的表达变化,发现DsMPK还受到氧化胁迫的抑制.进而分析DsMPK在高温、低盐、氧化、低温、高盐和紫外胁迫中翻译和磷酸化修饰的变化,发现除低盐胁迫外,在磷酸化修饰水平DsMPK同样受到各种非生物胁迫的抑制.在对不同生长阶段盐藻DsMPK磷酸化变化的分析中还发现,DsMPK的磷酸化水平与生长速率有一致性.各方面DsMPK的变化趋势说明其为一调控生长相关的MAPK.Mitogen-activated protein kinase（MAPKs） cascades play key roles in cell development,proliferation,and differentiation of all eukaryote under biotic and abiotic stresses.The microalgae Dunaliella salina is a model for studying tolerance to various abiotic stresses,and it performs excellent in its adaptation to the hypersalinity environment and other abiotic stresses.Previously,from D.salina we had indentified DsMPK,which was repressed on transcription level under cold and hypersalinity condition.In this study,the transcription of DsMPK was analyzed under heat,hypersalinity,UV and oxidative stresses.The transcription of DsMPK also decreased under oxidative stress.The DsMPK profiles of translation and phosphorylation were detected by western blotting.On phosphorylation level,DsMPK was repressed under cold,heat,UV and oxidative stresses,but not under hyposalinity stress.Furthermore,the DsMPK profile of phosphorylation was correlated with the change of growth rate during D.salina culture.It was proposed that DsMPK might mediate a signal transduction of cell division.

关 键 词：盐生杜氏藻 促有丝分裂活化蛋白激酶 非生物胁迫 信号调控 磷酸化修饰 

分 类 号：Q945.78[生物学—植物学] Q78