东亚飞蝗辅酶Q-细胞色素C还原酶基因克隆和序列分析  

cDNA Cloning and Sequence Analysis of Ubiquinol-cytochrome C Reductase Gene from Locusta migratoria manilensis

作　　者：司风玲[1] 何正波[1] 赵杨[1] 陈斌[1] 

机构地区：[1]重庆师范大学生命科学学院,昆虫与分子生物学研究所,重庆高校生物活性物质工程研究中心,重庆高校动物生物学重点实验室,重庆400047

出　　处：《应用与环境生物学报》2011年第1期78-81,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30700435);重庆市自然科学基金重点项目(No.CSTC2008BA5030)资助~~

摘　　要：辅酶Q-细胞色素C还原酶是组成线粒体呼吸链的4种酶复合体之一,对线粒体呼吸链的电子传递起着重要作用.同时,辅酶Q-细胞色素C还原酶还是杀真菌剂(Fungicide)、抗原虫(Antiprotozoan)、抗癌药物(Anticancer drugs)以及抗疟疾(Antimalarial)药物——阿托喹酮(Atovaquone)、氯胍(Proguanil)、布帕伐醌(Buparvaquone)的作用靶标,可将它作为昆虫的药靶,设计和开发新型的杀虫剂.采用RACE方法,克隆了东亚飞蝗[Locusta migratoria manilensis(Meyen)]辅酶Q-细胞色素C还原酶cDNA全序列(GenBank登录号:GU593056.1).获得的cDNA全长939 bp,其中可读框819 bp,编码272个氨基酸,推测其相对分子质量为29.48 ku,等电点为9.19.通过与其它10个物种的细胞色素C还原酶基因的氨基酸序列进行比对,发现东亚飞蝗与家蚕、赤拟谷盗、黒腹果蝇、埃及伊蚊、致乏库蚊、小家鼠、人、丽蝇蛹集金小蜂、蜜蜂和豌豆蚜的辅酶Q-细胞色素C还原酶氨基酸序列同源性分别为72%、72%、66%、63%、60%、59%、59%、58%、57%和56%.Ubiquinol-cytochrome C reductase is one of the four components of the mitochondrial respiratory chain,and plays an important role in the electron transfer of the mitochondrial respiratory chain.In addition,it acts as novel target of some drugs,so it has the potential as an insect drug target for design and development of new pesticides,such as fungicide,antiprotozoan,anticancer drugs,atovaquone,proguanil and buparvaquone.In this study,the full cDNA of ubiquinol-cytochrome C reductase gene from Locusta migratoria manilensis（Meyen） was cloned by means of 3′-RACE and 5′-RACE.The results showed that the cDNA was 939 bp long and contained an open reading frame（ORF） of 819 bp that encoded 272 amino acids with a calculated molecular weight of 29.48 ku and an isoelectric point of 9.19（GenBank accession number： GU593056.1）.Comparing the amino acid sequence of ubiquinol-cytochrome C reductase from L.migratoria manilensis with others from Bombyx mori,Tribolium castaneum,Drosophila melanogaster,Aedes aegypti,Culex quinquefasciatus,Mus musculus,Homo sapiens,Nasonia vitripennis,Apis mellifera and Acyrthosiphon pisum,the similarity is 72%,72%,66%,63%,60%,59%,59%,58%,57% and 56%.These data may facilitate the studies of ubiquinol-cytochrome C reductase function in insects and its application in developing new pesticides.

关 键 词：东亚飞蝗 辅酶Q-细胞色素C还原酶基因 RACE 序列分析 

分 类 号：Q78[生物学—分子生物学]