5味收涩药对奶牛乳腺炎病原菌体外抗菌活性的比较  

Comparative study on antibacterial activity in vitro of 5 kinds of astringent therapy herbs against pathogenic bacteria from dairy cow with mastitis

作　　者：代敏[1,2] 彭成[1] 万峰[1] 陈丹丹[1] 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室,成都610075 [2]成都医学院,成都610083

出　　处：《中国乳品工业》2011年第2期41-44,共4页China Dairy Industry

基　　金：国家"十一五"支撑重点项目(2009BAI84B04)

摘　　要：采用琼脂平板二倍稀释法测定了诃子等5味收涩药对15个种属33种120株奶牛乳腺炎病原菌的最低抑菌浓度,统计分析了它们对葡萄球菌、链球菌、大肠埃希菌、阴沟肠杆菌和单胞菌等共11个种属的MIC50和MIC90,并采用SPSS17.0软件中单因素方差分析法比较分析了5味收涩药分别对葡萄球菌、鲁氏不动杆菌和芽孢杆菌等11个种属菌株抑菌活性的强弱。结果表明,5味收涩药对受试菌均有较强的抗菌活性,总体以诃子的抗菌活性最强,其次为乌梅和石榴皮,再次为五味子,活性最差为山茱萸;且5味收涩药对革兰氏阳性菌的抗菌活性较对革兰氏阴性菌的活性强。The agar dilution assay was used to test the minimal inhibitory concentration for 5 astringent therapy herbs to 120 strains pathogenic bacteria from dairy cow with mastiffs. To compare antibacterial activity, one-factor analysis of variance（ANOVA）, MIC50 and MIC50 were used for statistical analysis. The results showed that 5 astringent therapy drugs had strong antimicrobial activity against 120 clinical isolates from cows. And Terrninalia chebula Potz had strongest antibacterial effects; dark plum and pomegranate peel had higher antibacterial effects; Schisandra had high antibacterial effects; comus officinalis had the lowest antibacterial activity. Furthermore, compared with gram-negitive germs, all drugs showed a strong antibacterial activity to gram-positive germs, and the experimental drug showed different antibacterial activity to different species of experimental bacteria.

关 键 词：体外抗菌活性 收涩药 乳腺炎病原菌 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]