HPLC测定蒙药曼陀罗子中东莨菪碱的含量  

Determination of Scopolamine in Datura stramonium by HPLC

作　　者：薛培凤[1] 陈玉荣[1] 青梅[1] 杨慧[1] 

机构地区：[1]内蒙古医学院药学院,呼和浩特010059

出　　处：《中国现代应用药学》2011年第2期164-167,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：内蒙古政府蒙药现代化重大研究专项蒙药材质量标准化资助项目(MX2005ZB015)

摘　　要：目的建立曼陀罗子中东莨菪碱的含量测定方法。方法色谱柱为Kromasil C18(250 mm×4.6 mm,5μm)。乙腈-水(含0.07 mol.L-1磷酸钠和0.017 5 mol.L-1十二烷基硫酸钠,0.5%磷酸,0.1%三乙胺)(37∶63)为流动相,检测波长为216nm。结果东莨菪碱在0.038 1～0.190 5 mg.mL-1内呈良好的线性,r=0.999 9;平均回收率为97.0%,RSD为0.93%。结论该方法简便、灵敏、准确、重复性好,可作为曼陀罗子的质量控制标准。OBJECTIVE To establish the method of determination of scopolamine in Datura stramonium.METHODS Kromasil C18（250 mm×4.6 mm,5 μm） column was employed.Mobile phase was the mixture of acetonitrile and Na3PO4 buffer（contains 0.07 mol·L.1 Na3PO4,0.017 5 mol·L.1 SDS,0.5% H3PO4,0.1% trithylamine）（37∶63）.RESULTS A good linearity of scopolamine was achieved in the range of 0.038 1.0.190 5 mg·mL.1（r=0.999 9）.The average recovery of scopolamine was 96.97% and RSD was 0.93%.CONCLUSION This method is simple,sensitive,accurate with good reproducibility,and can be used for the quality control of Datura stramonium.

关 键 词：曼陀罗子 东莨菪碱 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]