依那普利、厄贝沙坦及血管紧张素-(1-7)对快速心房起搏犬血管紧张素转换酶的影响  

Effects of enalapril,irbesartan and angiotensin-(1-7) on the expression of angiotensin converting enzyme in atrial tachypacing dogs

作　　者：李杨[1] 王学文[1] 李健[1] 徐昭[1] 李广平[1] 

机构地区：[1]天津医科大学第二医院心脏科天津心脏病学研究所,天津300211

出　　处：《中国心脏起搏与心电生理杂志》2011年第1期65-67,共3页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：国家自然科学基金项目(项目编号:30770863)

摘　　要：目的观察快速心房起搏后局部血管紧张素转换酶(ACE)和血管紧张素转换酶2(ACE2)表达的变化,以及应用依那普利、厄贝沙坦及血管紧张素-(1-7)[Ang-(1-7)]对其影响。方法健康成年杂种犬30只,分为假手术组(S组),心房起搏对照组(C组),心房起搏+依那普利组(EN组),心房起搏+厄贝沙坦组(IB组),心房起搏+Ang-(1-7)组(A组),每组6只。所有犬均安置起搏器。除假手术组外,其余各组均给予500次/分持续心房起搏刺激2周。EN组及IB组于起搏开始前3天开始给予口服依那普利2 mg.kg-1.d-1或厄贝沙坦60 mg.kg-1.d-1,A组经A lze t渗透泵连续给予Ang-(1-7)6μg.kg-1.h-1。2周后取右房肌组织,利用免疫组织化学方法检测心房肌组织中ACE和ACE2的蛋白表达情况。结果 C组较S组ACE表达显著升高,ACE2表达显著降低(P均<0.01),应用依那普利、厄贝沙坦和Ang-(1-7)可明显上调ACE2表达(与C组比较,P<0.05),Ang-(1-7)同时可下调ACE的表达(与C组比较,P<0.05)。结论 Ang-(1-7)可有效逆转起搏时的ACE/ACE2比例升高。Objective To investigate the expression of angiotensin converting enzyme （ACE） and ACE2 in an atrial tachypacing model of dogs and the effects of enalapril, irbesartan and angiotensin-（1-7） on ACE and ACE2 expression. Methods Thirty mongrel dogs were assigned to the sham-operated group （ S）, pacing-control group （ C）, pacing and enalapril-treated group （ EN）, pacing and irbesartan-treated group （IB） or pacing and Ang-（ 1-7 ） -treated group （A）, 6 dogs in each group. The rapid atrial pacing at 500 beats per minute was maintained for 2 weeks except sham group. The dogs in the EN, IB and A groups respectively received enalapril （2 mg · kg ^-1· d ^-1 ）, irbesartan （60 mg · kg ^-1· d^-1） orally or Ang-（1-7） （6 μg · kg^-1 ·h^-1） intravenously via mini-osmotic pumps during the pacing period. Immunohistochemical staining was applied to assess the protein expression of ACE and ACE2 in right atrial tissue. Result Compared with S group, ACE expression was markedly increased and ACE2 was markedly declined in C group （P 〈0. 01 ）. Enalapril, irbesartan or angiotensin-（1-7） treatement caused ACE2 expression increased （ P 〈 0.05 vs C group）. Angiotensin-（ 1-7 ） also downregulated the ACE expression （P 〈 0.05 vs C group）. Conclusion Angiotensin-（ 1-7 ） can inhibit the increased ACE/ACE2 ratio induced by atrial pacing.

关 键 词：心血管病学 心房颤动 血管紧张素-(1-7) 血管紧张素转换酶 

分 类 号：R541.75[医药卫生—心血管疾病] R972.2[医药卫生—内科学]