微核试验和彗星试验观察职业性二甲基乙酰胺暴露的遗传损伤  

Detection of Genetic Damages of Occupational Dimethylacetamide Exposure with Micronucleus Test and Comet Assay

作　　者：周连芳[1] 寿卫国[1] 吴国华[1] 薛振宇[1] 

机构地区：[1]杭州市萧山区疾病预防控制中心职业卫生监督科,浙江杭州311201

出　　处：《环境与职业医学》2011年第2期98-99,共2页Journal of Environmental and Occupational Medicine

摘　　要：[目的]观察二甲基乙酰胺(DMAc)对职业接触人群所产生的遗传损伤。[方法]用胞质阻断微核试验(CBMN)和彗星试验(SCGE)检测某氨纶企业DMAc接触工人(30名)及非接触DMAc的对照人员(30名)的外周血淋巴细胞DNA和染色体损伤。[结果]接触组微核率、微核细胞率、核分裂指数分别为(4.67±2.25)‰、(4.56±2.19)‰、(1.82±0.30)‰,对照组分别为(5.00±2.62)‰、(4.89±2.59)‰、(1.73±0.25)‰,两组的各指标间差异均无统计学意义(P>0.05)。接触组彗星尾DNA百分比、细胞尾长、尾相分别为(4.76±2.63)%、(12.60±5.68)μm、(2.51±2.30),对照组分别为(4.49±2.48)%、(11.77±5.01)μm、(2.28±1.89),各指标间的差异均无统计学意义(P>0.05)。[结论]在本实验条件下,职业性DMAc接触人群外周血淋巴细胞的染色体和DNA未表现出遗传损伤。[ Objective ] To study the genetic damages of occupational exposure to Dimethylacetamide（ DMAc ）. [ Methods ] The damages of DNA and chromosomes of peripheral blood lymphocytes were determined by comet assay and cytokinesis-block micronucleus test in exposed group consisting of 30 workers occupationally exposed to DMAc and a control group consisting of 30 workers without exposure to DMAc. [ Results ] In the exposure group, the micronucleus rate, micronucleus cell rate, and nuclei division index were（ 4.67±2.25 ）‰,（ 4.56±2.19 ）‰,（ 1.82 ±0.30 ）‰, and showed no statistical significance of difference between those versus to the control group [ （ 5.00±2.62 ） ‰, （ 4.89±2.59 ）‰, （ 1.73±0.25 ）‰] （ P 〉 0.05 ）. The Tail DNA %, mean tail length, and meadians of mean tail moment were （4.76±2.63 ）%, （ 12.60±5.68 ）μm, （2.51±2.30 ）, and showed no statistical significance difference between those to the control group [ （ 4.49±2.48 ） %, （ 11.77±5.01 ） μm, （ 2.28±1.89 ） ]（ P〉 0.05 ）. [ Gonclusion ] Under condition in this experiment, DMAc occupational exposure exhibited no damage effect on DNA and chromosomes on workers.

关 键 词：二甲基乙酰胺 DNA损伤 职业暴露 

分 类 号：R114[医药卫生—卫生毒理学]