栽培西陵知母中菝葜皂苷元含量动态研究  

Study on Dynamic Variation of Sarsasapongenin Content in Cultivated Xiling Zhimu from Yi County

作　　者：赵兵[1] 陈千良[1] 倪士峰[1] 王文全[2] 马长华[2] 刘建勇 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,陕西西安710069 [2]北京中医药大学中药学院,北京100102 [3]易县科技局,河北易县074200

出　　处：《辽宁中医药大学学报》2011年第3期22-24,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金："十五"国家重大科技专项项目(2001BBA701A62-13);教育部博士点新教师基金项目(20096101120013);陕西省中医药管理局科研项目(zy56);西北大学科研启动项目(okyqdf0159)

摘　　要：目的:研究栽培西陵知母中菝葜皂苷元的积累变化规律。方法:采用高效液相蒸发光散射检测,测定不同采集时间知母药材中菝葜皂苷元的含量。色谱柱:Kromasil C18柱(250mm×4.6mm,5μm)。流动相:甲醇,流速1mL.min-1。柱温为30℃。蒸发光散射检测器漂移管温度为50℃,蒸发温度为70℃,以氮气为雾化气,压力为1.03×105Pa。采用胆固醇为内标物,内标法定量。结果:栽培西陵知母各月菝葜皂苷元含量变化较大,尤以6月为最高,7月最低。春季各月含量总体平均高于6月份以后各月。结论:采收期对西陵知母药材质量有较大影响。栽培西陵知母中菝葜皂苷元的含量春季相对较高,春季知母返青前采挖,药材质量较高。Objective：Study on dynamic variation of sarsasapongenin content in cultivated Xiling Zhimu（Anemarrhena Rhizome）from Yi County to investigate its quality.Method：HPLC-ELSD method was adopted to determine the content of sarsasapongenin.With cholesterol as internal standard,Kromasil C18 column（250mm×4.6mm,5μm）was used.Mobile phase was methanol with the flow rate of 1mL·min-1;the temperature of the drift tube and evaporation was 50℃ and 70℃ respectively.The gas pressure was 1.03×105 Pa.Results：The sarsasapongenin content in samples collected in different month varied greatly.Sample collected in June has the highest content,while sample collected in July has the lowest.Samples of spring have a higher average content than those collected after June.Conclusion：The collection time has a great effect on quality of crude drug.Contents of sarsasapongenin in cultivated Anemarrhena Rhizome from Yi County are relative higher in spring.The best harvesting time is early spring.

关 键 词：西陵知母 菝葜皂苷元 HPLC-ELSD 含量动态 

分 类 号：R284.2[医药卫生—中药学]