2008-2009年北京市沙门菌流行特征和分子分型  

Epidemiological characteristics and molecular typing of Salmonella in Beijing from 2008 to 2009

作　　者：曲梅[1] 黄芳[1] 张新[1] 张代涛[1] 刘桂荣[1] 崔志刚[2] 严寒秋[1] 刘元[1] 何战英[1] 窦相峰[1] 阚飙 王全意[1] 

机构地区：[1]北京市疾病预防控制中心传染病地方病控制所,100013 [2]中国疾病预防控制中心传染病预防控制所

出　　处：《中华预防医学杂志》2011年第2期113-117,共5页Chinese Journal of Preventive Medicine

基　　金：国家高技术研究发展计划（2008AA02Z416）;“十一五”国家科技重大专项（2008ZX10004-008）;北京市科技新星计划（2008B28）

摘　　要：目的 分析2008-2009年北京市沙门菌流行特征及脉冲场凝胶电泳（PFGE）分子分型. 方法 对2008-2009年通过WHO全球沙门菌监测系统及北京市肠道门诊监测系统分离到的137株沙门菌进行生化鉴定、血清分型和相关流行病学分析;利用PFGE进行分子分型. 结果 北京市2008-2009年沙门菌的流行具有明显季节性,6-9月份高发,共分离菌株84株,占64.1%（84/131）;患者年龄多为18～40岁,占46.1%（58/128）;男性80例,女性51例,男女比例为1.57：1.137株沙门菌分属于20种血清型,其中肠炎沙门菌和鼠伤寒沙门菌为优势菌型,分别占46.7%（64/137）和17.5%（24/137）.共分71种PFGE带型,其中肠炎沙门菌和鼠伤寒沙门菌都有16种PFGE型别.肠炎沙门菌的4种PFGE型别（JEGX01.CN0001、JEGX01.CN0003、JFGX01.CN0002、JEGX01.CN0019）和鼠伤寒沙门菌的JPXX01.CN0001为优势分子型别. 结论 2008-2009年北京市沙门菌的流行具有性别、年龄和季节性分布特征;PFGE分子型别较多,且存在差异明显的多个克隆系.Objective To study the epidemiological characteristics and molecular phenotypes of Salmonella by pulsed-field gel electrophoresis （PFGE） in Beijing from 2008 to 2009. Methods A total of one hundred thirty-seven isolates recovered from the WHO Global Samonella Surveillance system and entero clinic surveillance system were identified by biochemical tests and serotyping.The related epidemiological informations were also analyzed.The isolates were further typed by PFGE. Results The prevalence of Salmonella from 2008 to 2009 showed obvious seasonal character.High incidence occurred from June to September,and 64.1% （84/131） isolates were recovered in this period.Patients of 18-40 year-old were 46.1% （58/128） and 80 patients were male and 40 patients were female with the ratio of 1.57：1.These 137 Salmonella isolates belonged to 20 serotypes,including Enteritidis （46.7%,64/137） and Typhimurium （17.5%,24/137） as the dominant serotype.In total,71 PFGE profiles were identified.Four PFGE patterns of S.Enteritidis isolates （JEGX01.CN0001,JEGX01.CN0003,JEGXO1.CN0002,JEGX01.CN0019） and S.Typhimurium pattern of JPXX01.CN0001 were dominant patterns. Conclusion The prevalence of Salmonella from 2008 to 2009 showed distribution characteristics of sex,age and seasons.The numerous PFGE patterns of Salmonella showed diversity of these isolates and different clones existed in Beijing.

关 键 词：沙门菌属 电泳 凝胶 脉冲场 细菌分型技术 

分 类 号：R378[医药卫生—病原生物学]