高效液相色谱法测定川东獐牙菜中獐牙菜苦苷的含量  

Determination of Swertiamarin in Swertia davidi Franch.by HPLC

作　　者：蔡兴东[1] 杨建平[1] 黄春[1] 沈力[1] 李润琴[1] 刘丹[1] 张乐星[1] 

机构地区：[1]重庆三峡医药高等专科学校,重庆万州404020

出　　处：《中国医药导报》2011年第5期42-43,共2页China Medical Herald

基　　金：重庆市教育委员会科学技术研究项目资助(No:KJ082102)

摘　　要：目的:建立川东獐牙菜中獐牙菜苦苷的含量测定方法。方法:采用高效液相色谱法,Crest C18(4.6 mm×250 mm,5μm)色谱柱,流动相:甲醇-水(25∶75),流速:1 ml/min,检测波长:254 nm,柱温:25℃。结果:獐牙菜苦苷在0.4～2.0μg范围内线性关系良好(r=0.999 9),平均回收率为99.78%,RSD=1.86%(n=6)。结论:该方法简便、准确,重现性好,可作为川东獐牙菜中獐牙菜苦苷的含量测定方法。Objective： To establish a method for the determination of Swer-tiamarin in Swertia davidi Franch.by HPLC.Methods： High performance liquid chromatography was performed on C18 column.The chromatographic conditions were as follows： methyl alcohol-water（25∶75） as mobile phase,flow rate being 1.0 ml/min,the detecting wavelength at 254 nm and column temperature being 25℃.Results： Swertiamarin had good linear relation within the range from 0.4 to 2.0 μg（r=0.999 9）.The average recovery was 99.78% with a RSD of 1.86%（n=6）.Conclusion： This method is convenient,accurate,reproducible,and can be used for content determinaton of Swertiamarin in Swertia davidi Franch..

关 键 词：川东獐牙菜 獐牙菜苦苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]