大孔吸附树脂分离纯化黄连小檗碱研究  

Study on separation and purification of berberine in Coptis chinensis by macroporous adsorption resin

作　　者：席国萍[1] 宋国斌[1] 

机构地区：[1]山西大同大学医学院,山西大同037009

出　　处：《中国医药导报》2011年第5期44-46,共3页China Medical Herald

摘　　要：目的:筛选适用于分离纯化黄连小檗碱的大孔吸附树脂。方法:本文考察了10种大孔吸附树脂,筛选出了适用于分离纯化黄连小檗碱的D101大孔吸附树脂,其静态吸附量为18.08 mg/g,静态和动态解吸率均为95%以上。结果:选用D101大孔吸附树脂,先水洗除杂,再用4～5倍树脂体积的60%乙醇洗脱,浓缩干燥,产品中小檗碱的含量为30.02%,产品收率为9.12%。结论:本法简单、易行,适合黄连小檗碱的提取。Objective： To study on separation and purification of berberine in Coptis chinensis by macroporous adsorption resin.Methods： From the 10 kinds of different macroporous adsorption resins,the D101 resin which the adsorption performance was 18.08 mg/g and the desorption ratio was over 95% was selected to separate the berberine in Coptis chinensis.Results： The desorption ratio of over 95% could be gotten by using 60% ethanol as eluting solvent and 4-5 BV.Under these experiment conditions,the content of berberine in products was 30.02% and the yield was 9.12%.Conclusion： The method is easy,possessed of popularization and application value.It can be used for separation berberine from Coptis chinensis.

关 键 词：黄连 小檗碱 分离 纯化 

分 类 号：R284.2[医药卫生—中药学]