亮菌防治顺铂化疗诱发胃肠道反应的机制研究  

An experimental study of the mechanisms of Armillariella tabescens for the prevention of cisplatin-induced gastrointestinal tract reaction in the rat

作　　者：杜静[1] 李平[1] 张梅[1] 孙鑫[1] 靳福鹏[1] 

机构地区：[1]安徽医科大学附属省立医院中医科,安徽合肥230001

出　　处：《安徽医药》2011年第2期144-147,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家中医药管理局资助项目(No2009J30)

摘　　要：目的探讨亮菌对顺铂化疗诱发胃肠道反应的防治机制。方法 90只雄性SD大鼠随机分为6组:空白对照组、模型组、亮菌低、中、高剂量组及昂丹司琼组,腹腔注射顺铂制造化疗性大鼠异食癖恶心呕吐模型,采用ELISA法测定大鼠胃肠及脑组织中5-HT含量,以观察亮菌对化疗后大鼠组织中5-HT含量的影响。结果造模后24、48及72 h,亮菌高、中剂量组大鼠高岭土摄入量均显著低于模型组(P<0.05);造模后24 h亮菌中剂量组、造模后24、48、72 h亮菌高剂量组的十二指肠与胃窦组织5-HT含量均显著低于模型组(P<0.01),而延髓组织5-HT含量仅表现为造模后24 h亮菌高剂量组显著低于模型组(P<0.05)。结论亮菌可有效的抑制顺铂化疗诱发大鼠高岭土异食癖反应,其作用机制可能与亮菌可抑制化疗后大鼠胃肠道组织5-HT的释放有关。Aim To study of the mechanisms of of Armillariella tabescens for the prevention of chemotherapy-induced gastrointestinal tract reaction by cisplatin.Methods Ninety male Sprague-Dawley rats were randomized into control group,model group,low dose of Armillariella tabescens group,middle dose of Armillariella tabescens group,high dose of Armillariella tabescens group and ondansetron group.Rats were injected intraperitoneally with cisplatin to induce pica,Using ELISA technique to assay the content of 5-HT in gastrointestinal tract and brain tissue,and observe the effect of Armillariella tabescens on 5-HT.Results 24～72 h after cisplatin administration,in the middle and high dose of Armillariella tabescens group,kaolin intake lower significantly compared to intake in the model group,respectively（P0.05）.After cisplatin administration,5-HT in duodenum and gastric antrum tissue,the middle dose of Armillariella tabescens group at 24 h（P0.01）and the high dose of Armillariella tabescens group during 24～72 h（P0.01）was all significantly lower than the model group,respectively,but 5-HT in pons medulla tissue,only the high dose of Armillariella tabescens group at 24 h（P0.05）was significantly lower than the model group.Conclusion Armillariella tabescens could effectively inhibit cisplatin-induced pica response of Kaolin,and the mechanisms of Armillariella tabescens preventing cisplatin-induced pica response should be that it might inhibit the release of 5-HT in duodenum and gastric antrum tissue.

关 键 词：亮菌 化疗 高岭土 5-HT 

分 类 号：R285.5[医药卫生—中药学]