蚕丝蛋白和明胶复合组织工程材料的组织相容性研究  

Histocompatibility of Silk/Gelatin Composite Scaffolds

作　　者：杨照[1] 申少波[2] 刘文晶[3] 徐丽莎[1] 赵倩[4] 韩英[1] 尹芳[1] 周新民[1] 

机构地区：[1]第四军医大学西京消化病医院国家肿瘤生物学重点实验室,陕西西安710032 [2]第四军医大学军事预防医学系卫生勤务教研室 [3]第四军医大学医学教育教研室 [4]西安交通大学机械学院

出　　处：《华南国防医学杂志》2011年第1期44-47,共4页Military Medical Journal of South China

基　　金：国家863计划(2009AA043801)

摘　　要：目的研究蚕丝蛋白-明胶三维材料支架,在SD大鼠体内所引起的免疫排斥反应,以及随着时间的降解情况。方法将3种不同比例总共90个蚕丝蛋白-明胶三维材料支架,植入3组共45只雄性SD大鼠(200～250g)背部皮下,每组15只大鼠,每只大鼠背部均匀植入3个同种比例材料,分别于植入后第7天、第14天、第30天处死各组内5只大鼠,取出被皮下纤维包裹的材料,4%多聚甲醛固定后行蜡块包埋,切片后进行HE染色。结果随着明胶成份在复合材料中所占比例增加,材料在大鼠体内的降解明显增快,3种比例材料第7天HE切片染色均显示较少单核细胞及中性粒细胞等炎性细胞渗出;第14天HE切片染色显示单核细胞及中性粒细胞等炎性细胞伴少量成纤维细胞渗出,材料开始出现降解;第30天材料大部分被组织机化、降解,大量成纤维细胞包绕材料残片。结论蚕丝蛋白与明胶复合材料支架具有良好的组织相容性和一定的降解能力,通过改进,在肝组织工程应用方面将具有一定应用前景。Objective To testify the immunological rejection of silk/gelatin composite scaffolds and its degradation in SD rats. Methods Male SD rats （200-250g） were anesthetized by intraperitoneal injections of sodium pentobarbital for subcutaneous pockets madding and 3D silk fibroin/gelatin scaffolds （10 mm diameter×1mm thickness） implantation. A total of 90 scaffolds were implanted in 45 rats （3 groups for 3 blends of scaffolds, 15 rats in each group）. Five rats in each group were sacrificed at the 7th, 14th and 30th day. The scaffolds and the surrounding tissues were extracted, chemically fixed for 4 hours with 10% formalin, paraffin embedded, and cut into sections with 5μm thickness for HE staining. Results As the ratio of gelatin increased, the degradation of composite scaffolds became faster. On the 7th day, all the scaffold sections showed exudation of some monocytes and neutrophils. On the 14th day, exudation of some monocytes, neutrophils and fibroblasts were observed and the scaffold began to degrade. On the 30th day, most of the scaffolds were degraded and surrounded by the fibrous tissue. Conclusion The scaffold composed of silk protein materials and gelatin has good histocompatibility and degradation ability. It will be useful in liver tissue engineering.

关 键 词：蚕丝蛋白 明胶 肝组织工程 组织相容性 

分 类 号：Q816[生物学—生物工程]