测定种子生活力方法的比较与改进  

Comparison and improvement of three kinds of methods to determine seed viability

作　　者：刘亚丽[1] 赵喜亭[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《实验技术与管理》2011年第1期46-48,共3页Experimental Technology and Management

基　　金：河南省自然科学基金(0511020600)

摘　　要：用氯化三苯基四氮唑(TTC)法、红墨水法和溴麝香草酚蓝(BTB)法测定种子生活力,对实验选用的种子、浸泡时间、TTC的药液配置浓度、红墨水染色法染色时间、BTB法加入琼脂浓度及琼脂凝胶层厚度作了改进。结果表明:缩短了3种方法测定种子生活力的时间,且效果更好,测定的准确率更高;TTC染色法红色更深,红墨水染色法种胚与胚乳更易分辨。The 2,3,5,-Triphenyl tetrazolium chloride,red ink and Bromothymol blue were used to determine the viability of seed.The method of selecting seed,time of soaking,destiny of 2,3,5,-Triphenyl tetrazolium chloride density of Bromothymol blue put in Agar powder,thickness of agar powder were improved.The result showed that the time for determining seed viability was shortened and better effect was obtained,and the accuracy was higher.

关 键 词：种子生活力 氯化三苯基四氮唑法 红墨水法 溴麝香草酚蓝法 

分 类 号：S339.3[农业科学—作物遗传育种]