环境水样中生化需氧量的光度法测定研究  

Study on the determination of BOD_5 in water sample by spectrophotometry

作　　者：沙鸥[1] 顾叶华[1] 孙梅[1] 马卫兴[1] 郭妍 

机构地区：[1]淮海工学院化学工程学院环境工程系,江苏连云港222005 [2]连云港金兆水务有限公司,江苏连云港222005

出　　处：《冶金分析》2011年第1期50-54,共5页Metallurgical Analysis

基　　金：江苏省高校自然科学研究计划资助项目(09KJD430002);江苏省自然科学基金项目(SBK200930560);淮海工学院自然科学研究课题资助项目(Z2007045)

摘　　要：对传统的生化需氧量(BOD)测定方法进行了改进,采用I3--结晶紫-聚乙烯醇体系光度法测定样品培养前后的溶解氧浓度,实验以碘酸钾与过量碘化钾生成单质碘,新生成的I3-与结晶紫生成的离子缔合物在550nm处有最大吸收,采用光度法测定5天前后培养水样中溶解氧的含量来计算水样中BOD5。所拟方法用于BOD5标准样品溶液、葡萄糖-谷氨酸标准溶液和污水厂进出口水样中BOD5的测定,结果与国家标准方法所得结果一致。The conventional determination method for biochemical oxygen demand（BOD） was improved.With I3^—methyl violet-polyvinyl alcohol spectrophotometric system,the concentration of dissolved oxygen before and after sample treatment was determined.Potassium iodate and excessive potassium iodide generated I3^-which formed with methyl violet an ion complex with maximum absorption at 550 nm.BOD5 could be worked out with the content of dissolved oxygen determined by spectrophotometry before and after 5 days treatment.The proposed method was applied to the determination of BOD5 in standard sample solution,glucose-glutamic acid standard solution and the influent and effluent water from waster water factory.The results were consitent with those by national standard method of China.

关 键 词：生化需氧量 光度法 结晶紫 

分 类 号：X832[环境科学与工程—环境工程]