枸杞优选优株快繁体系的建立及优质种苗繁殖技术研究  

A Study on the Establishment of Rapid Propagation System and Propagation Techniques of High-quality seedling of Wolfberry

作　　者：廖晴[1] 玛尔哈巴[1] 沙红[2] 高燕[2] 赵玉玲 

机构地区：[1]新疆农业科学院园艺作物研究所,乌鲁木齐830091 [2]新疆农业科学院经济作物研究所,乌鲁木齐830091 [3]新疆精河县枸杞管理中心,新疆精河833300

出　　处：《新疆农业科学》2011年第1期35-38,共4页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区林业科技财政专项资金项目"精河枸杞产业关键技术研究"

摘　　要：[目的]以枸杞宁杞1号优选优株嫩枝为外植体，研究影响枸杞离体快繁的各种因素，建立优选优株快繁体系，以期达到大量培育枸杞优质种苗的目的。[方法]通过观察采集优选优株，进行外植体的灭菌方法、侧芽诱导及继代培养、生根培养基激素的筛选，确定出相适应的培养基；同时对试管苗的移栽基质也进行了筛选。[结果]加用青霉素注射液500mg／L浸泡5—8min后进行外殖体进行表面消毒时，易获得无菌外殖体。适宜培养基：侧芽诱导MS＋6-BA0．5mg／L＋IAA0．1mg／L；增殖培养基为MS＋6-BA0．5mg／L＋IAA 1．0mg／L；生根培养基1／2MS＋IBA 0．3mg／L；移栽基质泥炭：珍珠岩2：1，成活率96％。[结论]枸杞优选优株快繁体系的建立，对于加速育种进程和新育良种的繁育、推广奠定了良好的技术基础，通过优选优株进行枸杞优质种苗的培育是可行的。[Objective] The wolfberry Ningji las explants, the superior strains of rapid propagation of optimization system were built by studying effect factors of rapid propagation. [ Method ] Excellent plants were selected by observing the collection line; the method of sterilization of explants, lateral bud induction and subculture, rooting hormone were screened to identify corresponding culture medium; while the transplanted matrix of plantlets in the test tube were screened also. [Result]The results showed： when explants were disinfected, 500 mg/ L penicillin should be used by soaking for 5 - 8 rain. Optimization medium were： buds were induced by medium： MS ＋ 6 - BA 0.5 mg/L ＋ IAA0. 1 mg/L;regeneration medium： MS ＋ 6 - BA 0.5 mg/L ＋ IAA 1.0 mg/L;root was induced by medium： 1/2MS ＋ IBA 0.3 mg/L; the transplanted matrix was peat： perlite 2： 1, the surviving rate was 96%. [Conculsion] The establishment of rapid propagation system of woli＇berry hish- guality seedlinss laid the foundations of accelerating the propagation and extension of new varieties.

关 键 词：枸杞 组织培养 繁殖 内生菌 

分 类 号：S663.9[农业科学—果树学] S603.6[农业科学—园艺学]