地衣共生菌分离培养的新方法  

New method for isolation and culture of lichenized fungi

作　　者：邱振鲁[1] 王海英[1] 赵遵田[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《山东科学》2011年第1期33-35,共3页Shandong Science

基　　金：济南市科技局(200914006)

摘　　要：介绍了一种分离培养地衣共生菌和内生菌的新方法。该方法直接挑取地衣体髓层菌丝进行培养,PDA培养基25℃培养14 d后观察,发现共生菌菌落生长率80.43%,而杂菌污染率为7.14%,具有简单、高效和污染率低的优点。This paper presented a new method for isolation and culture of lichenized fungi and lichen endophyte fungi. We directly selected medulla mycelium for cultivation with PDA nutrition medium. The growth rate of the community of lichenized fungi was 80.43%, while the contamination rate was only 7.14% under the conditions of nutrition medium of 25~C and 14 days later. The superiority of this method was therefore simplicity, high-efficiency and low contamination rate .

关 键 词：地衣共生菌 地衣内生菌 分离培养 

分 类 号：Q949.34[生物学—植物学]