草鱼PPARα和PPARγ基因的克隆与组织表达差异  

Cloning of PPARα and PPARγ Partial cDNA Sequence and Differential Expression in Tissues in Grass Carp(Ctenopharyngodon idella)

作　　者：林亚秋[1] 吉红[2] 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]西北农林科技大学动物科技学院水产系,陕西杨凌712100

出　　处：《水产科学》2011年第2期94-97,共4页Fisheries Science

基　　金：国家自然科学基金资助项目(30771667)

摘　　要：根据GenBank上登录的过氧化物酶体增殖物激活受体s(Peroxisome proliferator activated receptor,PPARs)基因序列设计2对引物,提取草鱼肝脏组织总RNA,经RT-PCR扩增获得了PPARα624 bp和PPARγ438 bp。并利用半定量RT-PCR分析了草鱼PPARα和PPARγ基因的mRNA组织表达特性,结果在肝胰脏、脂肪、肌肉、肠、脑、性腺、鳔、肝胰脏、心脏、脾脏和鳃均检测到PPARα和PPARγ基因的表达,在黏液中未检测到表达。且PPARα在肝胰脏中表达最高,PPARγ在脂肪组织中表达最高。Two primers of PPARα and PPARγ were designed according to gene sequence data of PPARs（peroxisome proliferator activated receptor,PPARs） in Genbank.Total RNA was extracted from the hepatopancreas of grass carp（Ctenopharyngodon idella）,and 624 bp and 438 bp PPARα and PPARγ gene was obtained by RT-PCR.The expression profiling of PPARα and PPARγ in the tissues was analyzed using semi-quantitative RT-PCR.PPARα and PPARγ expression was observed in adipose tissue,muscle,intestine,brain,gonad,swim bladder,hepatopancreas,heart,spleen and gill,except for mucilage.The maximal expression level of PPARα was found in hepatopancreas,while that of PPARγ was found in adipose tissue.

关 键 词：PPARΑ PPARΓ 克隆 组织差异 草鱼 

分 类 号：Q785[生物学—分子生物学]