块根紫金牛总黄酮提取工艺优化和不同产地、部位的含量比较  

Optimization of Extracting Conditions and the Content Comparative Analysis of Flavonoid of Ardisia corymbifera Mez var. tuberifera C. Chen from Different Areas and Different Sections

作　　者：罗宝丽[1,2] 蒋运生[1] 唐辉[1] 韦记青[1] 史艳财[1] 邹蓉[1] 韦霄[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,桂林541006 [2]广西师范大学生命科学学院,桂林541004

出　　处：《基因组学与应用生物学》2011年第1期57-61,共5页Genomics and Applied Biology

基　　金：广西科技攻关项目(桂科攻0992003B-37);广西植物研究所基本业务费项目(桂植业10001);广西科技创新能力建设项目(桂科能0992028-10)共同资助

摘　　要：本研究采用正交试验分析和分光光度法,建立了块根紫金牛总黄酮含量测定的方法并比较分析其不同产地及不同部位总黄酮的含量差异。结果表明超声波法提取块根紫金牛中总黄酮的最佳提取工艺为:乙醇浓度60%,料液比1:70,提取时间50min,提取功率200W,提取温度70℃。另外,不同产地块根紫金牛中总黄酮含量顺序为:靖西>那坡>德保>扶绥>龙州>武鸣;同一产地块根紫金牛根、茎、叶间总黄酮含量的高低顺序大致为:叶>根>茎。且通过精密度、重复性、稳定性和回收率实验来检测最佳提取工艺条件,结果显示该方法操作简便,结果准确,重复性好,RSD为0.788%,稳定性和回收率较高,平均回收率达96.20%。证明该方法可用于比较不同产地块根紫金牛药材中总黄酮的含量差异,对不同产地块根紫金牛的药材质量分析具有一定的指导作用。In this research, we established a method of quantity determination of total flavonoid in Ardisiacorymbifera Mez var. tuberifera C. Chen and comparative analysed content differences of flavonoid in different sections A. var. tuberifera from different areas by orthogonal experiment and spectrophotometry. The results indicated the optimum extractive technology of determination of total flavonoid in A. var. tuberifera by spectrophotometry was that extraction reagent 60% alcoho1, ratio of liquid/solid as 1：70, extracting power 200 W, extracting temperature 70℃ and extracting time 50 min. On the other hand, the quantitative order of flavonoid of A. var. tuberifera from different areas was that JingxiNapoDebaoFusuiLongzhouWuming as well as the quantitative order of flavonoid in different sections of A. var. tuberifera from the same area was leafrootstem. The optimum extract condition was detected by the experiment of precision, repeatability, stability and recovery, The results displayed that this method was simple, accurate, repeatable, the RSD was 0.788%, stable and high recovery with the average recovery of 96.20%, which showed that this method was feasibling for comparative content differences of flavonoid in different sections A. var. tuberifera from different areas and would provide some important direction for its quality analysis.

关 键 词：块根紫金牛 总黄酮 提取工艺 含量测定 

分 类 号：S567.239[农业科学—中草药栽培]