中国美利奴羊PRLR基因外显子10 SSCP多态性与部分繁殖性状的关联分析  

Relationship between Polymorphism of Exon 10 of Prolactin Receptor Gene and Reproduction Trait of Chinese Merion Sheep

作　　者：吴洪宾[1] 吴荷群 王遵宝[1] 余鹏[3] 赵宗胜[1] 张文祥[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]新疆兵团农六师五家渠市畜牧兽医工作站,五家渠831300 [3]石河子大学生命科学学院,石河子832003

出　　处：《石河子大学学报（自然科学版）》2011年第1期45-49,共5页Journal of Shihezi University(Natural Science)

摘　　要：以催乳素受体基因(PRLR)作为绵羊繁殖性状的候选基因,运用PCR-SSCP方法对PRLR外显子10的多态性进行检测,并对其多态性与绵羊部分繁殖性状进行了关联分析。结果表明:在PRLR外显子10的2个基因片段存在PCR-SSCP多态,PRLR1扩增片段存在3种基因型,PRLR2扩增片段存在4种基因型;Hardy-Weinberg平衡检验显示,中国美利奴羊群体在2个位点都处于平衡状态;CE和DD基因型中国美利奴羊产羔数均值比CD和DE型多0.31只(P<0.05);AA基因型中国美利奴羊初生重均值比BB型重0.66 kg(P<0.05);DE基因型中国美利奴羊3月龄重均值比CD型重2.42 kg(P<0.05)。This study selected prolactin receptor（PRLR） as the candidate gene of sheep reprodution trait,detected the polymorphism of exon 10 of prolactin receptor gene and analyzed the relationship between polymorphism of exon 10 of PRLR between reproduction trait of sheep.The result showed that there were two transitions in exon 10 of sheep PRLR gene,which were three genetypes of PRLR1 and four genetypes of PRLP2;both of the two sites accorded with Hardy Weinberg equilibrium in Chinese Merino sheep.The correlation analysis showed that the Chinese Merino sheep with genotype CE and DD had 0.31（P0.05） lambs more than those with genotype CD or DE;the average birth weight of genetype AA was 0.66 kg heavier than genetype BB（P0.05）;the average three-month weight of genetype DE was 2.42 kg heavier than genetype CD（P0.05）.

关 键 词：绵羊 PRLR 繁殖性状 PCR-SSCP 

分 类 号：S827.2[农业科学—畜牧学] Q959.842[农业科学—畜牧兽医]