正交试验法优选丁香叶中原儿茶酸的提取工艺  

An Extraction Technology of Optimization of ProtocatechuicAcid from Syringa Oblate Leaves by Orthogonal Experiment

作　　者：张蓓[1] 班维固[1] 潘慧莹[1] 许贵军[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2011年第1期54-56,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江中医药大学科研基金项目(编号:200918)

摘　　要：目的:优选丁香叶中原儿茶酸的最佳提取工艺。方法:以原儿茶酸为评价指标成分,采用高效液相色谱法测定丁香叶中原儿茶酸的含量,并用L9(34)正交试验法考察加水量、提取时间、提取次数等因素的影响,优化提取条件。结果:最佳提取工艺为加20倍量水,1.5h提取2次,70%醇沉24h后乙酸乙酯萃取。结论:优选得到的工艺稳定、合理、可行。Objective： To optimize the extraction technology of protocatechuic acid in Syringa Oblate Leaves.Methods： An L9（34）orthogonal experiment was performed to investigate the 3 factors（3 levels for each factor were selected）,water,extraction time,and extraction number with the protocatechuic acid as indexes for evaluation,which was determined by HPLC.Result： The optimum extraction process was 20 times of water,extracting 2 times,every 1.5h,70% alcohol precipitated for 24h,then extracted by ethyl acetate.Conclusion： The optimized technology for the extraction of protocatechuic acid from Syringa Oblate Leaves is stable,reasonable and feasible.

关 键 词：丁香叶 原儿茶酸 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]