桑树枝干乳汁中的1-脱氧野尻霉素含量测定与分析  

Determination and Analysis of 1-Deoxynojirimycin Content in Latex of Mulberry Branch

作　　者：张和禹[1] 汪泰初[2] 刘金珠[1] 胡娟霞[1] 刘朝良[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽省农业科学院蚕桑研究所,合肥230061

出　　处：《蚕业科学》2011年第1期121-124,共4页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系建设专项(蚕桑)

摘　　要：桑树等桑科植物的乳汁中主要含有生物碱等化学物质。为筛选乳汁中富含1-脱氧野尻霉素(DNJ)的药用桑树材料,采用柱前衍生化反相高效液相色谱-紫外检测法,测定不同桑树品种及桑树不同部位、不同季节采集乳汁中的DNJ含量。不同桑树品种枝条中部乳汁中的DNJ含量有较大差异,其中华桑种的金寨华桑的DNJ含量高于鲁桑种的黄鲁选品种3倍之多。同一桑树品种(农桑14号)的桑树不同部位及不同收获季节采集乳汁中的DNJ含量也有差异:夏季采集的枝条中部乳汁中的DNJ质量比为6.338 mg/g,枝条顶部乳汁中的质量比为5.813 mg/g,主干基部乳汁中的质量比仅为1.638 mg/g;夏季枝干乳汁中的DNJ含量极显著高于冬季枝干乳汁中的含量。Latex of mulberry trees and other plants of Moraceae family mainly contains alkaloids and other chemical substances. In order to screen medicinal mulberry materials of which latex has rich content of 1-deoxynojirimycin （DNJ）, a pre-column derivatization reverse-phase high-performance liquid chromatography and UV detection method was employed to determine DNJ content in latex collected from various mulberry varieties, from different portions of mulberry tree, and in various seasons. It was found that different mulberry varieties had great difference in DNJ content of latex collected from middle portion of branch, among which DNJ content in Jinzhaihuasang （ Morus cathayana） was 3 times higher over that in Huangluxuan （Morus multicaulis）. In the same mulberry variety （Nongsang 14）, DNJ content in latex collected from different portions of the tree and in different seasons was also varied. DNJ content in latex from the middle portion of mulberry branch collected in summer was 6. 338 mg/g. That from the upper portion of mulberry branch was 5. 813 mg/g. And that from the lower portion of mulberry trunk was only 1. 638 mg/g. DNJ content in latex of mulberry branch and trunk in summer was significantly higher than that in winter.

关 键 词：桑树 乳汁 1-脱氧野尻霉素 品种 树体部位 收获季节 

分 类 号：S888[农业科学—特种经济动物饲养]