油橄榄叶中羟基酪醇含量及提取工艺研究  

Content Determination and Extraction Technology of Hydroxytyrosol from Olive Leaves

作　　者：叶建中[1,2,3,4] 王成章[1,2,3,4] 陈虹霞[1,2,3] 董艳鹤[1,2,3] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2011年第1期63-67,共5页Chemistry and Industry of Forest Products

基　　金：中国林科院林业新技术研究所基本科研业务费专项基金(CAFINT2008C04;CAFINT2010K05);农业科技成果转化资金项目(2010GB24320626)

摘　　要：油橄榄含有橄榄苦苷、黄酮类和羟基酪醇等多酚抗氧化物。采用ODS(4.6 mm×200 mm,5μm)色谱柱,紫外检测波长为230 nm,甲醇-0.2%醋酸水溶液6∶94(体积比)为流动相,流速1 mL/min,建立羟基酪醇的HPLC分析方法,对不同品种的油橄榄叶进行含量分析。结果表明:10个品种油橄榄叶中羟基酪醇质量分数范围在0.3%～0.8%之间,小莱星含量最高,配多灵含量最低。通过单因素和正交试验研究了超声波提取油橄榄叶中羟基酪醇的工艺,1 g油橄榄叶粉末正交试验的优化结果为:功率120 W、90%甲醇提取、料液比1∶20(g∶mL)、时间20 min。采用90%甲醇提取油橄榄叶,提取液经AB-8大孔树脂、硅胶柱层析和HPLC制备色谱,分离质量分数95%以上的单体,通过MS、NMR鉴定该化合物为羟基酪醇。Many polyphenolic, antioxidants such as oleuropein, flavonoids and hydroxytyrosol exist in olive leaves. The identification of hydroxytyrosol in different breeds of olive leaves was carried out by HPLC moritored at 230 nm with ODS column （ φ 4.6 mm × 200 mm, 5 μm） , MeOH-O. 2 % acetic acid buffer 6 ： 94 （ volume ratio） as mobile phase at flow rate 1 mL/min. The contents of hydroxytyrosol were distributed from 0.3 % to 0.8 % in 10 breeds. The ultrasonic extract of hydroxytyrosol was studied by single factor and orthogonal experiment. The optimum extraction conditions were as follows：ultrasonic power 120 W, 90 % MeOH-H20 as extractant, material-liquid ratio 1 ： 20 （g： mL） , extraction time 20 min. Meanwhile, a single compound of 95 % content was seperated by AB-8 maeroporous resin, silica gel column chromatography and preparative HPLC. It was identificated to be hydroxytyrosol by MS and NMR.

关 键 词：油橄榄叶 羟基酪醇 正交试验 化学结构 

分 类 号：TQ351.0[化学工程]