大豆蛋白质含量QTL的“整合”及Overview分析  

Integration of QTLs Related to Soybean Protein Content and "Qualification" of Them by Overview Method

作　　者：刘硕[1,2] 罗玲[1] 刘章雄[1] 关荣霞[1] 邱丽娟[2] 

机构地区：[1]南昌大学生命科学学院,江西南昌330031 [2]中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《大豆科学》2011年第1期1-7,14,共8页Soybean Science

基　　金：国家重点基础研究发展计划资助项目(2009CB118403);国家转基因专项资助项目(2008ZX08009-003);国际科技合作计划项目(2008DFA330550)

摘　　要：在物理整合的基础上,选用元分析方法将已发表的与蛋白质相关的QTL整合到物理整合中重复次数较多的6个QTL区间处,并且选用基于统计学原理的Overview方法进行优化,得到连锁群上QTL的有效遗传位置,最终筛选出稳定性较强的标记Satt127,研究结果为标记辅助鉴定提供了理论依据。Soybean protein is the main source of vegetable protein.Developing high protein content soybean is one of main aims in soybean breeding.Up to now,lots of QTLs have been revealed related to soybean protein content,but due to the different populations,markers and environments,the QTLs have not been integrated systematically.This study mainly referred to the physical integrations of QTL results and select meta-analysis to confirm 6 stable QTL regions.Moreover,overview method based on statistic thesis was also chosen for qualifications of QTLs and confirmations of valid QTLs positions.Finally,the most stable SSR marker Satt127 had been screened out,which could apply evidence for marker-assisted breeding.

关 键 词：大豆 蛋白质 物理整合 元分析 OVERVIEW 优化 

分 类 号：S565.1[农业科学—作物学]