6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响  

Effect of Different 6-BA Concentration and Genotypes on Shoots Induced from Embryonic Tips

作　　者：闫帆[1] 孙昕[1] 翟莹[1] 李晓薇[1] 王英[1] 李景文[1] 王庆钰[1] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062

出　　处：《大豆科学》2011年第1期29-32,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2008ZX08004-003);国家自然科学基金面上资助项目(30971808);吉林省科技发展计划重点资助项目(20080204);长春市科技局国际科技合作资助项目(08GH10);"211工程"三期重点学科建设资助项目

摘　　要：以吉林35、吉林47等5个大豆品种为材料,胚尖为外植体,研究不同浓度6-BA对丛生芽诱芽率和平均芽数的影响,同时测定吉林35在胚尖诱导丛生芽的过程中对潮霉素的耐受性。结果表明:3.0 mg.L-16-BA浓度下吉林35的诱芽率最高,综合考虑丛生芽诱芽率和平均芽数2个性状,吉林35更加适合于大豆胚尖再生系统;在遗传转化中,以3.0 mg.L-1潮霉素作为吉林35抗性筛选的适宜浓度。In order to establish an efficient embryonic tip regeneration system for soybean（Glycine max L.Merr.）,five soybean cultivars,including Jilin 35,were used as explants to study the effects of 6-BA and genotypes on the numbers and induction rate of adventitious shoots.The endurance of the induced shoots of Jilin 35 to Hygromycin B was also studied.The results showed that Jilin 35 was fit for soybean embryonic tip regeneration system according to the numbers and inductivity of adventitious shoots.The concentration of 3.0 mg·L-1 Hygromycin B was advised for resistance screening in embryonic tip regeneration system.

关 键 词：大豆 胚尖 6-BA 基因型 

分 类 号：S565.1[农业科学—作物学]