利用^(15)N标记确定氮素营养水平对大豆籽粒氮素构成的影响  

Effects of Nitrogen Nutrition Levels on Nitrogenous Constitution of Soybean Seeds by ^(15)N Labeling

作　　者：董守坤[1] 刘丽君[1] 张冰[1] 马春梅[1] 龚振平[1] 祖伟[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2011年第1期92-95,共4页Soybean Science

基　　金：国家"十一五"科技支撑计划资助项目(2007BAD89B05);黑龙江省科技攻关资助项目(GA09B103;GA09B104);东北农业大学科技人才启动基金资助项目(2009RC57)

摘　　要：以东农47为试材,采用15N标记的(NH4)2SO4为氮源,利用砂培方式研究了氮素营养水平对大豆籽粒氮素构成和产量的影响。结果表明:随外源氮水平增加,大豆产量呈先增加后降低的变化趋势,营养液中氮为50 mg.L-1时产量最高;前期高氮后期低氮处理时,随高氮处理的时间延长产量降低;于R4或R5期提高外源氮水平可以增加大豆产量。对大豆产量与籽粒中肥料氮所占比例、大豆产量与根瘤固氮所占比例分别作回归分析,经求导分析得出大豆籽粒中外源氮所占比例为39.1%、根瘤固氮所占比例为61.5%时大豆产量最高。由此推断,当外源氮对籽粒的贡献率为40%左右、根瘤固氮贡献率为60%左右时,能够同时满足大豆对根瘤固氮和外源氮的需求,有利于大豆产量的提高。Labeled 15N ammonium sulfate was used as nitrogen source to study the effects of nitrogen nutrition levels on soybean（cultivar Dongnong 47） nitrogenous constitution and yields by sand culture.The results were shown as following： the crop yield increased at first and decreased later as the exogenous-N level heightened.When the nitrogen concentration was 50 mg·L-1,crop yield was the highest.Heightened exogenous nitrogen level between R4 and R5 could increase yield of soybean.The regression between yield and proportion of fertilizer-N in seed,yield and proportion of nodulation-N were analyzed.The result of derivation analysis showed that highest yield could be obtained when the proportion of soil-N and nodulation-N was 39.1% and 61.5%,respectively.We could deduce that when the contribution rate of exogenous nitrogen and nodulation-N was about 40% and 60% in seeds,respectively,these would satisfy the requirement of soybean seeds and benefit soybean yield formation.

关 键 词：大豆 外源氮 15N标记 根瘤固氮 产量 

分 类 号：S565.1[农业科学—作物学]