大豆异黄酮提取-测定优化体系的建立  

Establishment of an Optimized System about Extraction-Determination of Soybean Isoflavone

作　　者：张艳[1] 李晓薇[1] 张海军[1] 张庆林[1] 翟莹[1] 钱丹丹[1] 晏晓峰[1] 王庆钰[1] 李景文[1] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062

出　　处：《大豆科学》2011年第1期141-143,146,共4页Soybean Science

基　　金：转基因生物新品种培育重大专项子课题(2008ZX08004-003);国家自然科学基金面上项目(30971808);吉林省科技发展计划重点项目(20080204)

摘　　要：利用超声波法提取大豆异黄酮,结合三波长紫外分光光度法测定大豆异黄酮含量,对大豆异黄酮提取方法进行优化。结果获得的最优提取条件为:料液比1∶20、乙醇浓度50%、提取时间30 min,提取2次;并用三波长法测定24个大豆品种异黄酮含量,发现品种间异黄酮含量有显著差异,其中吉林32和吉林35含量最高,分别达到了0.292%和0.259%。The method to extract soybean isoflavone by ultrasonic wave was optimized,the content of which was determined by three-wave length UV spectrohotometry,in order to develop a high performance extraction-determination method of isoflavones in soybean.Results showed that the optimized condition were as follows： the ratio of material to solvent was 1∶20,concentration of ethanol was 50%,extraction time was 30 min,with 2 replicates.Twenty-four soybean varieties was mesured,their contents varied significantly.The content of soybean isoflavones was highest in Jilin 32 and Jilin 35,0.292% and 0.259%,respectively.

关 键 词：大豆异黄酮 超声波 三波长 优化体系 

分 类 号：TQ461[化学工程—制药化工]