己烯雌酚免疫抗原的合成与鉴定  

Synthesis and Identification of Artificial Antigens of Diethylstibestrol

作　　者：李利东[1] 宓晓黎[1] 黄丽俊[1] 袁建兴[1] 杜姝莲[1] 

机构地区：[1]江苏省微生物研究所有限责任公司,江苏无锡214063

出　　处：《动物医学进展》2011年第2期29-32,共4页Progress In Veterinary Medicine

基　　金：国家863高新技术发展计划课题(2008AA10Z425);江苏省科技支撑计划(BE2008631);无锡市科技支撑计划(CSE00804)

摘　　要：通过重氮化法引入羧基,再用碳二亚胺法偶联BSA获得己烯雌酚(DES)免疫抗原。经紫外光谱、电泳图谱分析确证获得DES-BSA偶联物。间接酶联免疫法分析证明其免疫鼠所获得的抗体与己烯雌酚有良好的特异性。The immunogen DES-BSA（diethylstibestrol-bovine serum albumin） were synthesized by the carbodilimide method through introduction of a carboxyl group into diethylstilbestrol by diazotization method.The results from UV,SDS-PAGE and ELISA showed that DES-BSA were conjugated successfully.The indirect ELISA results showed that antibodies derived from mice were specific to diethylstilbestrol.

关 键 词：己烯雌酚 人工抗原 重氮化 碳二亚胺法 

分 类 号：Q579.13[生物学—生物化学]