7种美白化合物对皮肤黑素细胞的体外生物学效应  

Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro

作　　者：张玮[1] 高静[2] 张敏[1] 王晓宇[1] 侯雪梅[1] 高申[1] 

机构地区：[1]第二军医大学附属长海医院药学部,上海200433 [2]第二军医大学药剂教研室,上海200433

出　　处：《药学实践杂志》2011年第1期24-26,40,共4页Journal of Pharmaceutical Practice

基　　金：国家自然基金:项目编号:NO.81000689;"重大新药创制"科技重大专项(2008ZXJ09010-001);上海市自然基金:项目编号10ZR1437300

摘　　要：目的研究7种美白化合物对体外培养的皮肤黑素细胞的生物学效应。方法利用B-16小鼠黑素瘤细胞株,以左旋多巴为底物测定细胞内酪氨酸酶活性,采用比色法测定细胞中黑色素含量,以溴化二苯四偶氮法(MTT法)测定化合物对黑素细胞活力的影响。结果各化合物对黑素细胞酪氨酸酶活性和黑素生成量呈浓度依赖性抑制,对黑素细胞活力均有不同程度的影响。结论这7种化合物均能抑制黑素细胞的黑色素生成。Objective To investigate the bio - efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B 16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lihteners could inhibit the melanocytes to produce melanin.

关 键 词：美白化学物 黑素细胞 黑色素 酪氨酸酶 

分 类 号：R945[医药卫生—微生物与生化药学]