3种肺炎支原体感染检测方法的临床效果比较  

Comparison of three methods for detection of Mycoplasma pneumonia

作　　者：王佳贺[1] 牛慧彦[1] 白雪[1] 李岩[1] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004

出　　处：《新乡医学院学报》2011年第1期51-53,共3页Journal of Xinxiang Medical University

基　　金：辽宁省教育厅资助项目(编号:L2010701)

摘　　要：目的比较实时荧光定量聚合酶链反应(FQ-PCR)法、培养-增强套式PCR(培养-增强nPCR)法和培养法检测肺炎支原体感染的临床应用价值。方法分别采用实时FQ-PCR法、培养-增强nPCR法和培养法对临床诊断为肺炎支原体肺炎和疑似肺炎支原体肺炎的97例患儿的咽拭子标本进行检测。结果实时FQ-PCR法检测的阳性率明显高于培养法(P<0.001),实时FQ-PCR法的检出率和培养-增强nPCR法比较无显著差别(P>0.05),但实时FQ-PCR法更加简便、快速。结论实时FQ-PCR法较培养-增强nPCR法和培养法更适用于肺炎支原体的早期快速诊断。Objective To compare the clinical application value of real-time fluorescence quantitation polymerase chain reaction（FQ-PCR）,cultured-enhanced nest PCR（nPCR） and mycoplasma culture in the detection of Mycoplasma pneumonia.Methods Pharyngeal swab specimen from 97 children（Mycoplasma pneumonia patients or Mycoplasma pneumonia-suspected patients）was detected by means of real-time FQ-PCR,cultured-enhanced nPCR and mycoplasma cultivation.Results The positive rate of detection with real-time FQ-PCR was significantly higher than that of mycoplasma cultivation（P0.001）.The positive rate was no significant difference between real-time FQ-PCR and cultured-enhanced nPCR（P0.05）.However,real-time FQ-PCR is much more convenient and quick than cultured-enhanced nPCR and culture method.Conclusion Real-time FQ-PCR is superior to cultured-enhanced nPCR and culture method for early diagnosis on Mycoplasma pneumonia infection.

关 键 词：肺炎支原体 实时荧光定量聚合酶链反应 套式聚合酶链反应 培养法 

分 类 号：R375[医药卫生—病原生物学]