磁场定位磁纳米化bFGF治疗大鼠腓肠肌钝挫伤恢复效果研究  

Recovery effectiveness of blunt trauma rat gastrocnemius treated by bFGF-coated magnetic nanoparticles

作　　者：董贵俊[1] 葛新发[1] 李可峰[1] 潘卫东[2] 

机构地区：[1]山东体育学院研究生部,山东济南250102 [2]中国科学院电工研究所生物医学工程部,北京100190

出　　处：《武汉体育学院学报》2011年第2期43-46,51,共5页Journal of Wuhan Sports University

基　　金：山东省自然科学基金(Y2007C116)成果

摘　　要：骨骼肌钝挫伤是影响运动员运动能力及运动寿命的一种常见疾病。在骨骼肌损伤修复过程中,碱性成纤维细胞生长因子(bFGF)骨骼肌肌原细胞有增殖作用,可以加快损伤肌肉修复。通过免疫化学偶联技术,制备重组bFGF包裹的磁性纳米粒,采用重物下落装置建立急性骨骼肌钝挫伤模型,在外加磁场的牵引下靶向聚焦至损伤部位。在损伤后第2、10、17、24和30天分别取样,测定和计算肌肉收缩力和应力松弛,并利用实时荧光定量PCR法测定大鼠腓肠肌MHC-Ⅱb mRNA的表达。结果发现,检测大鼠腓肠肌在受到钝挫伤后的第17天及第24天收缩力,磁纳米化bFGF组较bFGF组有显著性提高;在损伤后第2天,磁纳米化的bFGF即能使应力松弛恢复接近正常肌肉水平;与注射bFGF组相比,bFGF包裹磁性纳米粒对损伤肌肉收缩力的提高更明显。损伤后第2-10天,磁纳米化bFGF组与其余各组之间的MHC-Ⅱb mRNA表达差异有统计学意义。因此,磁纳米化的bFGF能明显改善大鼠急性骨骼肌钝挫伤后的收缩应力和恢复应力衰减,增强损伤肌肉MHC-Ⅱb mRNA的表达,更好地促进损伤肌肉修复。The purpose of this paper was to study the effects of basic fibroblast growth factor（bFGF）-coated magnetic nanoparticles on the recovery in rat skeletal muscles after acute contusion.Recombinant bFGF-coated magnetic nanoparticles were produced by immunochemical coupling techniques.The acute skeletal muscle contusion was produced by heavy objects dropping devices.Subcutaneous injection or blank control were performed respectively with each group and targeted and focused at the injured site with the guidance of external magnetic fields.The muscles were sampled at day 2,10,17,24 and 30 respectively after contusion,and then the muscle contractive strength and relaxation were measured and calculated.The expression of MHC-Ⅱb mRNA in rat gastrocnemius was investigated by real-time PCR assay.Magnetically nanocrystallized bFGF could significantly increase the muscle contractive strength at day 17 and day 24 in gastrocnemius after contusion.From day 2 after contusion,magnetically nanocrystallized bFGF could restore the muscle relaxation back close to the normal level.Compared with the direct injection bFGF group,BFGF-coated group more significantly increased the muscle contractive strength at day 17（p0.05）.BFGF-coated group was significantly different from other four groups in the expression of MHC-Ⅱb mRNA at day 2-10 after contusion（p0.05）.Magnetically nanocrystallized bFGF could significantly enhance the contractive strength,promote the expression of MHC-Ⅱb mRNA,restore the muscle relaxation and improve more effectively the healing of injured muscles in rat acute skeletal muscle contusion.

关 键 词：大鼠 腓肠肌 磁性纳米粒 碱性成纤维细胞生长因子 钝挫伤 

分 类 号：R873[医药卫生—运动医学]