反相高效液相色谱法测定人血吗氯贝胺浓度  

RP HPLC METHOD FOR DETERMINATION OF SERUM MOCLOBEMIDE CONCENTRATION

作　　者：傅崇铭[1] 柳福真[1] 郭瑞臣[2] 宫爱华[1] 王恩[1] 

机构地区：[1]山东省精神卫生中心 [2]山东医科大学附属医院

出　　处：《山东医科大学学报》1999年第3期261-262,共2页Acta Academiae Medicinae Shandong

摘　　要：以乙腈水（３０∶７０， Ｖ／ Ｖ）为流动相，氯霉素为内标，采用反相高效液相色谱法测定吗氯贝胺浓度。血吗氯贝胺在碱性条件下用乙醚提取，甲醇溶解残留物，在 ００４～４．０μｇ／ｍ ｌ浓度范围内线性关系良好，ｒ＝ ０．９９９６，相对平均回收率为 ９７０％ ，日内 Ｒ Ｓ Ｄ（％ ）２６～３．５，日间 Ｒ Ｓ Ｄ（％ ）３．７～４．２，最低检出浓度００４μｇ／ｍ ｌ。本法快速、准确，灵敏度高，专一性强，适用于吗氯贝胺临床监测及科研需要。To establish high pressure liquid chromatographic (HPLC) method for the determination of moclobemide concentrations, moclobemide in human serum was extracted with ester and separated on YWG C 18 colum with a mobile phase of acetonitrile:water (30∶70,V/V)and chloraphenicol was used as internal standerd. All detections occureed at 240nm. The linearity was excellent over the range of 0.04~4.0μg/ml(r=0.9996). The relative mean recovery of moclobemide was more than 97.0% and intra-day and inter-day RSD(%) were all less than 4.2. The minimum detection limit in serum was 0.04μg/ml.The method reported was rapid,simple, sensitive and of excellent precision and could be used in determination of serum moclobemide concentrations both for therapeutic drug monitoring and scientific studies.

关 键 词：吗氯贝胺 高压液相色谱法 血液浓度 

分 类 号：R969.1[医药卫生—药理学] R971.4[医药卫生—药学]